Hi sylwia. You really need to read the documentation we wrote for the coloc
2 plugin very carefully and also the papers hat are referenced there.
Fiji.sc/Coloc_2
PSF. Point spread function. The image of a point source of light is not a
point. This is explained in the Costes paper
The Costes randomization test is needed to tell you if the Pearson's and
Mander coefficients you measure are higher than a randomized version of
your image. If yes then good. If not then you have no better than random
colocalisation and should not infer any biological phenomenon.
Read all the docs. Read them again and the papers. Re read the docs. Digest
then new information. Then ask us more questions. It tool me months to
understand ....
Best
Dan
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