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Hello all imaje j users and Thanks Dan
I had another question about analysis of colocalization using JACoP.
i make substack of 4-5 slices for both the channels. Do i just use those substack as such for colocalization or do i merge these 4-5 slices in one image before i use them in JACoP.
If i use 4-5 slices for each substack, how does JACoP calculate the r value? Does it look at r value of each slice and then average it, or does it average out the pixel. i am so confused about how it calculates.
Gauri
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