Colocalization thresholds

[Giulia Falivelli]

Hi all,

  I just started using Image J and I am trying to do a colocalization
  analysis using the plugin "Colocalization thresholds". I used the z-stack
of
  my images (2 channels), but I don't fully understand all the output values
  that I have in the file Results.
  Can somebody help me to understand the meaning (or pointing me to the
right
  source) of: Rtotal, Rcoloc, R<threshold, M1, M2, tM1, tM2, Ncoloc.

  The tutorial that I found here
  http://pacific.mpi-cbg.de/wiki/index.php/ColocalizationAnalysis
   was not too helpful in understanding these values


  Thanks

  Giulia

[Gabriel Lapointe]

Personally, aside from the original papers, I Find that Tony Collins'
ImageJ for microscopy was most helpfull to understand those results.
http://www.macbiophotonics.ca/imagej/colour_analysis.htm

Good luck
Gabriel Lapointe

Giulia Falivelli wrote:

> Hi all,
>
>   I just started using Image J and I am trying to do a colocalization
>   analysis using the plugin "Colocalization thresholds". I used the z-stack
> of
>   my images (2 channels), but I don't fully understand all the output values
>   that I have in the file Results.
>   Can somebody help me to understand the meaning (or pointing me to the
> right
>   source) of: Rtotal, Rcoloc, R<threshold, M1, M2, tM1, tM2, Ncoloc.
>
>   The tutorial that I found here
>   http://pacific.mpi-cbg.de/wiki/index.php/ColocalizationAnalysis
>    was not too helpful in understanding these values
>
>
>   Thanks
>
>   Giulia
>
>