Comparison of fluorescence intensities

[Roenoeoe]

Hello everybody,

maybe somebody can help me solving the following problem with Fiji:

I have a fluorescence image of an embryo section and want to compare the
fluorescence intensities of two different structures.
Therefore I want to select both ROI and then compare them to get the
difference in fluorescence signal. So that I can state on different
expression levels in both structures.

Has anybody an idea how to do it?

Thanks in advance

[Peter Haub]

Hallo,

the ROI's will be selected manually?

Then open the ROIManager, select your ROI's, click <Add> to 'store'
every selected ROI.
Then simply click <Measure>.
(Select the features to be measured in <Analyze / Set Measurements / ..
Area .. Integrated Density .. Standrad Deviation .. etc.)

ph

On 18.07.2011 20:16, Roenoeoe wrote:

> Hello everybody,
>
> maybe somebody can help me solving the following problem with Fiji:
>
> I have a fluorescence image of an embryo section and want to compare the
> fluorescence intensities of two different structures.
> Therefore I want to select both ROI and then compare them to get the
> difference in fluorescence signal. So that I can state on different
> expression levels in both structures.
>
> Has anybody an idea how to do it?
>
> Thanks in advance
>
>
> --
> View this message in context: http://imagej.588099.n2.nabble.com/Comparison-of-fluorescence-intensities-tp6595791p6595791.html
> Sent from the ImageJ mailing list archive at Nabble.com.

[Roenoeoe]

hey,

the structures are in the same section and i select them manually.