Concentration calculation

[RajGourob Ghosh]

Dear All,

              I have to calculate the calcium conc. using the Grienkiwicz
equation, for a region of interest in FURA2 dual excited tiff images. how do
I go about using IMAGEJ. If anyone can provide a detailed explanation of the
equation as well as how to generate the values from the images it will be
very helpful  to me.

Rajgourav

[swaraj basu]

Dear Rajgourav,
                    Its is really difficult to calculate, I think your supervisor may be knowing.