Contrast applies to the entire stack?

[Andy Puckett]

Why must the settings of the Adjust>Brightness/Contrast dialog affect an entire image stack?  
Is there any way around this?  I have a set of 3 images which have different min/max pixel
values, and I would like to animate and analyze them as a stack, but there is no single
contrast setting that works for all three.  I would rather not fiddle with the values of the
pixels themselves, I consider this a display issue.

Thanks,
Andy

-------------------------------------------------------------
Dr. Andy Puckett [hidden email]
Postdoctoral Fellow 907-786-1838
University of Alaska Anchorage   SCI 221
-------------------------------------------------------------

[Josh Doe-2]

Andy,
As far as I know there is no way to do this, although I would be
interested if there is a way.

What I normally do is just run Enhance Contrast, but this does change
the pixel values, something you wish to avoid.

I'm not even sure how something like this would be implemented.

-Josh

On Wed, Mar 19, 2008 at 4:56 AM, Andy Puckett <[hidden email]> wrote:

> Why must the settings of the Adjust>Brightness/Contrast dialog affect an entire image stack?
>  Is there any way around this?  I have a set of 3 images which have different min/max pixel
>  values, and I would like to animate and analyze them as a stack, but there is no single
>  contrast setting that works for all three.  I would rather not fiddle with the values of the
>  pixels themselves, I consider this a display issue.
>
>  Thanks,
>  Andy
>
>  -------------------------------------------------------------
>  Dr. Andy Puckett                 [hidden email]
>  Postdoctoral Fellow                     907-786-1838
>  University of Alaska Anchorage     SCI 221
>  -------------------------------------------------------------
>

[Wayne Rasband]

> Why must the settings of the Adjust>Brightness/Contrast dialog affect
> an entire
> image stack? Is there any way around this?  I have a set of 3 images
> which have
> different min/max pixel values, and I would like to animate and
> analyze them as
> a stack, but there is no single contrast setting that works for all
> three.  I would
> rather not fiddle with the values of the pixels themselves, I consider
> this a
> display issue..

Use the Image>HyperStacks>Stack to HyperStack command to convert the
stack to a hyperstck. In the dialog box, set "Channels" to the number
of images in the stack and "Display Mode" to "Grayscale". In a
hyperstack, each channel has its own brightness and contrast settings.

-wayne