Correctly measure distance between three points

[Zazgah]

Hello,

I have very tiny cells (500 nm) marked with 2 or 3 fluorofores and I want to measure correctly the distance between them.

The only way I have found to do it is to stack the three fluorescence images in a Stack and then measure the distance with the plugin MtrackJ.

The problem I have is that the tool to track the points by the "Bright centroid" or "Maximum intensity" have a huge error for the resolution I need.

Is there a plugin or a way to measure distance between areas instead of points? Or a way to do it with points better?

Because I think I am getting a huge variability because of the non-exact measure of the center of the fluorescence signal.

Thank you in advance and sorry if the question is too basic

[BenTupper]

Hi,

On Jul 28, 2012, at 4:59 AM, Zazgah wrote:

> Hello,
>
> I have very tiny cells (500 nm) marked with 2 or 3 fluorofores and I want to
> measure correctly the distance between them.
>
> The only way I have found to do it is to stack the three fluorescence images
> in a Stack and then measure the distance with the plugin MtrackJ.
>
> The problem I have is that the tool to track the points by the "Bright
> centroid" or "Maximum intensity" have a huge error for the resolution I
> need.
>
> Is there a plugin or a way to measure distance between areas instead of
> points? Or a way to do it with points better?
>
> Because I think I am getting a huge variability because of the non-exact
> measure of the center of the fluorescence signal.
>
> Thank you in advance and sorry if the question is too basic

I don't think it is too basic, but I'm not sure what you are looking for either.  Here is a plugin that will measure distances between points or perimeters - but it is only 2D.  Could you modify the plugin for your needs?

http://rsb.info.nih.gov/ij/plugins/graph/index.html

Cheers,
Ben
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