Hello,
for a science project, I plan to quantify the number of fluorescence
foci per cell (lymphocytes) in some cell collectives. There are also
some granulocytes, monocytes and erythrocytes in the cell preparation,
which need to be excluded by size.
Currently, I take several overlapping pictures in 600x magnification
and stitch them with my microscopy software. A sample image can be
seen at <
http://www.geoforce.de/tog1.jpg>.
Since I need to count the foci in each cell in several time steps and
experiments, I would prefer some kind of automatic or semi-automatic
analysis.
Ideally, I could specify min and max diameter (calculated from
magnification) of the included cells and min/max diameter and min
brightness of the foci.
Is there a way to do this with ImageJ somehow?
Thank you very much for your help!
Dominik Geisel