Counting blood cells on a hemocytometer

[Gregor F Barclay]

I need a protocol for using Image J to count blood cells on a
hemocytometer. I have hundreds of counts to make, and I thought that IJ
would make this easy, but I cannot seem to find a way to threshold out
or otherwise make the grid lines insignificant enough that IJ sees just
the cells.

 

Any suggestions, especially  from someone who has done this, would be
appreciated. I can use Bright Field, Phase Contrast, DIC, or
epifluorescence microscopy to view the blood cells.

 

Greg Barclay

 

[hidden email]


--
We make a living by what we get, we make a life by what we give. - Sir
Winston Churchill

Dr. G. F. Barclay
Department of Life Sciences
University of the West Indies
St. Augustine
Republic of Trinidad and Tobago
868-662-2002 ext 3112

 

[lechristophe]

If you can have both bright field and fluo, maybe you can define the
area with the brightfield image (where you see the lines) and report
this ROI on the fluo image (where I assume you just see the cells), then
count inside this ROI.

Just a suggestion

Christophe

Gregor F Barclay wrote:

> I need a protocol for using Image J to count blood cells on a
> hemocytometer. I have hundreds of counts to make, and I thought that IJ
> would make this easy, but I cannot seem to find a way to threshold out
> or otherwise make the grid lines insignificant enough that IJ sees just
> the cells.
>
>  
>
> Any suggestions, especially  from someone who has done this, would be
> appreciated. I can use Bright Field, Phase Contrast, DIC, or
> epifluorescence microscopy to view the blood cells.
>
>  
>
> Greg Barclay
>
>  
>
> [hidden email]
>
>

... [show rest of quote]

[Joel Sheffield]

If you use the count particles routine, and select a specific range
of axial ratio, you should be able to eliminate the grid bars.  --
just a guess.  How about posting an image somewhere?


Date sent:       Tue, 28 Nov 2006 11:58:53 -0400
Send reply to:   ImageJ Interest Group <[hidden email]>
From:           Gregor F Barclay <[hidden email]>
Subject:         Counting blood cells on a hemocytometer
To:             [hidden email]

> I need a protocol for using Image J to count blood cells on a
> hemocytometer. I have hundreds of counts to make, and I thought that IJ
> would make this easy, but I cannot seem to find a way to threshold out
> or otherwise make the grid lines insignificant enough that IJ sees just
> the cells.
>
>  
>
> Any suggestions, especially  from someone who has done this, would be
> appreciated. I can use Bright Field, Phase Contrast, DIC, or
> epifluorescence microscopy to view the blood cells.
>
>  
>
> Greg Barclay
>
>  
>
> [hidden email]
>
>
> --
> We make a living by what we get, we make a life by what we give. - Sir
> Winston Churchill
>
> Dr. G. F. Barclay
> Department of Life Sciences
> University of the West Indies
> St. Augustine
> Republic of Trinidad and Tobago
> 868-662-2002 ext 3112
>
>  

... [show rest of quote]

--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
[hidden email]  
[phone], fax [phone]
http://astro.temple.edu/~jbs