Course Announcement-Final

[Hard, Robert]

This is the final announcement for the imaging course:


*Title:* Optical Microscopy and Imaging in the Biomedical Sciences


*When:* October 6 - October 15, 2009


*Where:* Marine Biology Laboratory, Woods Hole, MA, USA


*Tuition:* $2750 (Includes room and board, text, handouts, supplies)


*Application Deadline: *Applications accepted through June 26, 2009


*Admission application and information:*

Carol Hamel, Admissions Coordinator

Marine Biological Laboratory

7 MBL Street

Woods Hole, MA 02543-1015

[phone]

Internet: [hidden email]

WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)


*Course Directors: *


Robert Hard, University at Buffalo

Phone: [716] 829-3521

Email: [hidden email]


Colin S. Izzard, State University of New York @ Albany

Phone: [518] 442 - 4367

Email: [hidden email]


*Course Description:*


For Whom:

Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and digital imaging will benefit greatly from this course as
well. There are no specific prerequisites, but an understanding of the basic
principles of optics and imaging experience is desirable. Limited to 25
students. The nine day course consists of lectures, hands-on laboratory
exercises, problem sets, laboratory demonstrations and discussions that will
enable the participant to obtain and interpret microscope images of high
quality, to perform quantitative optical measurements, and to produce
digital records for documentation and analysis. For some structured
exercises, students, as small groups, will acquire, analyze, interpret and
present imaging data, thereby demonstrating their progress toward mastering
microscopic/imaging skills.


Live and fixed specimens will be provided for use in laboratory exercises
and  demonstrations.


HOWEVER, STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (PRIMARY
CULTURES, CELL LINES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS AND TO
USE THEM THROUGHOUT THE COURSE FOR EXERCISES, WHERE APPROPRIATE. STUDENTS
ALSO ARE ENCOURAGED TO MAKE APPOINTMENTS WITH OUR EXPERIENCED COMMERCIAL
FACULTY TO EXAMINE THEIR CELLS OUTSIDE OF SCHEDULED CLASSES USING THE STATE
OF THE ART EQUIPMENT AVAILABLE TO THEM. Cell culture facilities are
available for student use. Students are highly encouraged to discuss their
individual research problems with the academic and commercial faculty.


Topics to be covered include:


Principles of microscope design and image formation


Bright field, dark field, phase contrast, polarized light, differential
interference contrast, interference reflection, and fluorescence microscopy


Digital imaging, recording, enhancement, processing and analysis


Advanced fluorescent techniques (eg. FRAP, FLIP, FRET, FLIM, fluorescence
polarization,  multi-spectral and ratiometric imaging)


Confocal scanning microscopy, multiphoton excitation fluorescence microscopy
and  structured illumination systems


Image deconvolution, digital image restoration and 3-D reconstruction


3D image acquisition, display, and analysis


Live cell imaging


Laser microdissection and microcapture of tissues/cells for molecular
biology.


Super-resolution techniques (4PI, STED, PALM, etc.)


Applications to live cells will be emphasized; other specimens will be
covered as well.


Students will have direct hands-on experience with state-of-the-art
microscopes, digital cameras, photometers, and image
acquisition/processing/analysis software provided by major optical,
electronics, and software companies. Instruction will be provided by
experienced staff from universities and industry.



For faculty list and additional information, see:

http://www.mbl.edu/education/courses/special_topics/om.html