Density measurement ImageJ

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Density measurement ImageJ

Pieter Janssens
Hey guys,

I'm currently measuring the density of certain regions on rat brain slices (Western blot) in ImageJ.

But I was wondering, are there options to keep a drawn figure (for example with the polygon tool) fixed while drawing a new one?
Because, as you probably know, the last drawn selection always disappears when clicking next to it (to start drawing a new figure).

Another question: I know I can save my results (density measurements), but how do you open this data after closing and restarting ImageJ?

Thanks a lot for your help!

Greetings from Belgium,
Pieter

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Re: Density measurement ImageJ

Stoyan Pavlov
Hi Pieter,
If you hold Shift while drawing the second selection it is added to the
first without deleting it.
The results are saved in a text file, which is TAB-separated. It can be
opened with any text editor or it can be imported into any  spreadsheet
editor :MsOffice Excel, OpenOffice or LibreOffice Calc etc.

Stoyan Pavlov


2012/12/14 Pieter Janssens <[hidden email]>

> Hey guys,
>
> I'm currently measuring the density of certain regions on rat brain slices
> (Western blot) in ImageJ.
>
> But I was wondering, are there options to keep a drawn figure (for example
> with the polygon tool) fixed while drawing a new one?
> Because, as you probably know, the last drawn selection always disappears
> when clicking next to it (to start drawing a new figure).
>
> Another question: I know I can save my results (density measurements), but
> how do you open this data after closing and restarting ImageJ?
>
> Thanks a lot for your help!
>
> Greetings from Belgium,
> Pieter
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>



--
Dr. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
 Bulgaria
Tel: +359 (0) 52 - 677 - 086
e-mail: [hidden email]

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Re: Density measurement ImageJ

Stoyan Pavlov
Of course in this way your measurement will be done over the two drawn
areas at once. If you need to store them as separate selections you will
have to use the RoImanager: draw selection => hit Ctrl+T to add it as a
Region of interest and then repeat for all your RoIs. When you're done you
can save them and edit them with the RoImanager.


2012/12/14 Stoyan Pavlov <[hidden email]>

> Hi Pieter,
> If you hold Shift while drawing the second selection it is added to the
> first without deleting it.
> The results are saved in a text file, which is TAB-separated. It can be
> opened with any text editor or it can be imported into any  spreadsheet
> editor :MsOffice Excel, OpenOffice or LibreOffice Calc etc.
>
> Stoyan Pavlov
>
>
> 2012/12/14 Pieter Janssens <[hidden email]>
>
>> Hey guys,
>>
>> I'm currently measuring the density of certain regions on rat brain
>> slices (Western blot) in ImageJ.
>>
>> But I was wondering, are there options to keep a drawn figure (for
>> example with the polygon tool) fixed while drawing a new one?
>> Because, as you probably know, the last drawn selection always disappears
>> when clicking next to it (to start drawing a new figure).
>>
>> Another question: I know I can save my results (density measurements),
>> but how do you open this data after closing and restarting ImageJ?
>>
>> Thanks a lot for your help!
>>
>> Greetings from Belgium,
>> Pieter
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
>
>
> --
> Dr. Stoyan P. Pavlov, MD, PhD
> Departament of Anatomy, Histology and Embryology
> Medical University "Prof. Dr. Paraskev Stoyanov", Varna
> Prof. Marin Drinov Str.55
> 9002 Varna
>  Bulgaria
> Tel: +359 (0) 52 - 677 - 086
> e-mail: [hidden email]
>
>
>
>


--
Dr. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
 Bulgaria
Tel: +359 (0) 52 - 677 - 086
e-mail: [hidden email]

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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Re: Density measurement ImageJ

Rasband, Wayne (NIH/NIMH) [E]
In reply to this post by Pieter Janssens
On Dec 14, 2012, at 9:48 AM, Pieter Janssens wrote:

> Hey guys,
>
> I'm currently measuring the density of certain regions on rat brain slices (Western blot) in ImageJ.
>
> But I was wondering, are there options to keep a drawn figure (for example with the polygon tool) fixed while drawing a new one?

Press "b" (Image>Overlay>Add Selection) and the selection will be added to the overlay. Long click on a selection in the overlay to reactivate it.

> Because, as you probably know, the last drawn selection always disappears when clicking next to it (to start drawing a new figure).
>
> Another question: I know I can save my results (density measurements), but how do you open this data after closing and restarting ImageJ?

Use the File>Import>Results command.

-wayne
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