Detecting cell boundaries

[alisona5]

I have been trying to work on a protocol for automating muscle fiber measurements in ImageJ for a long time and getting nowhere.  There is a published protocol that uses the program CellP:

http://www.treat-nmd.eu/downloads/file/sops/dmd/MDX/DMD_M.1.2_001.pdf

In it they use a separator filter to detect cell boundaries because thresholding based on grey values does not work (pg 8).  It creates an image that converts the variable intensity cell boundaries to lines.  I have tried playing around with the filters in imageJ to duplicate this, but haven't come up with anything.  Does anyone have any ideas?
I greatly appreciate any help
Alison

[Nilwik Rachel (BW)]

Hi,

I have managed to create cell boundaries like the one in the article through Process-Binary-Skeletonize.
The image needs to be a binary image.

Doing this did not solve the whole problem for myself, though. I wanted to do Analyze Particles after this and that does not work because ImageJ does not recognize the cells as particles anymore... I was thinking that perhaps this can be solved by thickening the boudaries between the cells. But haven't figured how to do that...

Hope this ws of any use to you!
rachel