Hi Sara,
it is not clear to me how you want to combine the images, i.e., what
'overlap' means.
If you do an operation with the Process>Image Calculator, and 'Image 1'
is a stack, but Image 2 isn't, ImageJ will ask you whether to do the
operation for the whole stack.
So, you can e.g. add one image to all images of a stack, or get the
average of one image and each stack slice (i.e., blend the one image
with each stack slice, with equal weights of both).
You can also create a mask (for 8-bit or RGB images: 255=foreground,
0=background) and use the 'and' operation to set all stack slices to
zero in some region, then add another image that has nonzero pixels only
in that region.
In all these cases, you don't need to convert the image used as 'Image
2' in the Image Calculator to a stack.
Michael
________________________________________________________________
On 12/04/2017 14:02, Sara_T wrote:
> Hi,
>
> I currently have one snapshot (one frame) of an image of a cell. I wish to
> duplicate this one image, and then form a stack containing 50000 frames
> (50000 of the same image). Is there a possibility to do this kind of
> manipulation on imageJ?
>
> What I ultimately would like to do is this: I have a stack (stack-A) with
> 50000 frames. I also have an image (image-B) which is taken from exact same
> camera but has only one frame. I would like to overlap this image-B onto
> every frame in stack-A, to form a nice new stack (stack-C) in which I have
> stack-A overlapped with image-B in each frame.
>
> It would be great if some one could help me out!
>
> Thanks
>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html
Locked
