Extended Particle Analyzer - Different results on PC and Mac

[Straub, Volko A. (Dr.)]

Dear All,

I have observed some unexpected behaviour using the Extended Particle Analyzer in a macro.
I have analysed the attached image using the Extended Particle Analyzer as part of a macro with the following command:

min=20;
max=300;
run("Extended Particle Analyzer", "area="+min+"-"+max+" pixel roundness=0.4-1.00 show=Nothing redirect=None keep=None add reset");

This generates a list of 507 ROIs in the ROI manager.

However, when I gave the macro to a student for some analysis, who is using a Mac laptop, using the same command on the same image only found 287 ROIs. Manual inspection of the ROI table showed that many of the larger particles were missed.
I am stumped and have no idea why the same macro command applied to the same image should produce such different results just because the macro is executed on a different operating system.

Is this a bug? Any ideas what might be going on?
Thanks,
Volko






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[Aryeh Weiss]

You should check all of the Extended PA parameters, because the Extended
PA remembers parameters.
You might check the reset checkbox and then run it twice, on each machine.

--aryeh

On 05/02/2018 16:42, Straub, Volko A. (Dr.) wrote:

> Dear All,
>
> I have observed some unexpected behaviour using the Extended Particle Analyzer in a macro.
> I have analysed the attached image using the Extended Particle Analyzer as part of a macro with the following command:
>
> min=20;
> max=300;
> run("Extended Particle Analyzer", "area="+min+"-"+max+" pixel roundness=0.4-1.00 show=Nothing redirect=None keep=None add reset");
>
> This generates a list of 507 ROIs in the ROI manager.
>
> However, when I gave the macro to a student for some analysis, who is using a Mac laptop, using the same command on the same image only found 287 ROIs. Manual inspection of the ROI table showed that many of the larger particles were missed.
> I am stumped and have no idea why the same macro command applied to the same image should produce such different results just because the macro is executed on a different operating system.
>
> Is this a bug? Any ideas what might be going on?
> Thanks,
> Volko
>
>
>
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

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[ctrueden]

Hi Volko,

This FAQ entry might be helpful:
https://imagej.net/Troubleshooting#The_same_plugin_gives_different_results_on_different_machines.21

Regards,
Curtis

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LOCI software architect - https://loci.wisc.edu/software
ImageJ2 lead, Fiji maintainer - https://imagej.net/User:Rueden
Did you know ImageJ has a forum? http://forum.imagej.net/


On Mon, Feb 5, 2018 at 8:42 AM, Straub, Volko A. (Dr.) <[hidden email]
> wrote:

> Dear All,
>
> I have observed some unexpected behaviour using the Extended Particle
> Analyzer in a macro.
> I have analysed the attached image using the Extended Particle Analyzer as
> part of a macro with the following command:
>
> min=20;
> max=300;
> run("Extended Particle Analyzer", "area="+min+"-"+max+" pixel
> roundness=0.4-1.00 show=Nothing redirect=None keep=None add reset");
>
> This generates a list of 507 ROIs in the ROI manager.
>
> However, when I gave the macro to a student for some analysis, who is
> using a Mac laptop, using the same command on the same image only found 287
> ROIs. Manual inspection of the ROI table showed that many of the larger
> particles were missed.
> I am stumped and have no idea why the same macro command applied to the
> same image should produce such different results just because the macro is
> executed on a different operating system.
>
> Is this a bug? Any ideas what might be going on?
> Thanks,
> Volko
>
>
>
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[gankaku]

 Dear Volko,

Can you provide the complete macro?
My guess would be the black background definition (except the correct
particles are selected by the ROIs) or as Aryeh already stated any of the
other parameters. I personally cannot test it on a mac but have not seen
this behaviour before.

Regards,
Jan


2018-02-05 15:42 GMT+01:00 Straub, Volko A. (Dr.) <[hidden email]>:

> Dear All,
>
> I have observed some unexpected behaviour using the Extended Particle
> Analyzer in a macro.
> I have analysed the attached image using the Extended Particle Analyzer as
> part of a macro with the following command:
>
> min=20;
> max=300;
> run("Extended Particle Analyzer", "area="+min+"-"+max+" pixel
> roundness=0.4-1.00 show=Nothing redirect=None keep=None add reset");
>
> This generates a list of 507 ROIs in the ROI manager.
>
> However, when I gave the macro to a student for some analysis, who is
> using a Mac laptop, using the same command on the same image only found 287
> ROIs. Manual inspection of the ROI table showed that many of the larger
> particles were missed.
> I am stumped and have no idea why the same macro command applied to the
> same image should produce such different results just because the macro is
> executed on a different operating system.
>
> Is this a bug? Any ideas what might be going on?
> Thanks,
> Volko
>
>
>
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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phone: [phone]
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[gankaku]

Dear Volko,

I tested your macro and it works as intended and gives me 507 particles. If
the background definition is changed it leads to 0 results. So, currently I
do not have an Idea where the discrepancy of the values comes from.
Or did you meanwhile figure out what the problem was?
Would be important to know just in case it is a bug in the tool.

Kind regards,
Jan

2018-02-06 9:34 GMT+01:00 BioVoxxel <[hidden email]>:

> Dear Volko,
>
> Can you provide the complete macro?
> My guess would be the black background definition (except the correct
> particles are selected by the ROIs) or as Aryeh already stated any of the
> other parameters. I personally cannot test it on a mac but have not seen
> this behaviour before.
>
> Regards,
> Jan
>
>
> 2018-02-05 15:42 GMT+01:00 Straub, Volko A. (Dr.) <[hidden email]>:
>
>> Dear All,
>>
>> I have observed some unexpected behaviour using the Extended Particle
>> Analyzer in a macro.
>> I have analysed the attached image using the Extended Particle Analyzer
>> as part of a macro with the following command:
>>
>> min=20;
>> max=300;
>> run("Extended Particle Analyzer", "area="+min+"-"+max+" pixel
>> roundness=0.4-1.00 show=Nothing redirect=None keep=None add reset");
>>
>> This generates a list of 507 ROIs in the ROI manager.
>>
>> However, when I gave the macro to a student for some analysis, who is
>> using a Mac laptop, using the same command on the same image only found 287
>> ROIs. Manual inspection of the ROI table showed that many of the larger
>> particles were missed.
>> I am stumped and have no idea why the same macro command applied to the
>> same image should produce such different results just because the macro is
>> executed on a different operating system.
>>
>> Is this a bug? Any ideas what might be going on?
>> Thanks,
>> Volko
>>
>>
>>
>>
>>
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>
>
>
> --
>
> CEO: Dr. Jan Brocher
> phone: [phone] <+49%20176%2070574681>
> e-mail: [hidden email]
> info: [hidden email]
> inquiries: [hidden email]
> web: www.biovoxxel.de
>

--

CEO: Dr. Jan Brocher
phone: [phone]
e-mail: [hidden email]
info: [hidden email]
inquiries: [hidden email]
web: www.biovoxxel.de

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