Paxton and Folks
Looking at these fibers (electron-spun I assume),
I do not think that you have enough contrast
and separation to automeasure the diameter (sic).
You may want to freeze-fracture to check their
cross-sections for a non-unity aspect ratio.
Also, there has been a similar discussion on
the MSA listserver. You may want to post your
query there.
regards,
Jim
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> From: Paxton Provitera <
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> Subject: Fiber diameter revisited
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> Hi all.
> I am trying to determine fiber diameter from SEM images which have 100-200
> fibers of differeing diameters in random orientations.
> The perfect final output would be a list of diameters.
> I have looked through the available plugins and have not seen anything that
> jumps out as a solution, but before I begin writing a plugin I was hoping
> someone might have a better suggestion.
>
>
> Here's a link to a sample image:
>
http://paxtonprovitera.com/imageJ/SEM_sample.tif >
>
> Thanks for your help in advance.
>
> -Paxton
>
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