Fiji ImageJ rigid registration

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Fiji ImageJ rigid registration

pang
Dear all,

I would like to use Fiji ImageJ rigid registration, but I could not find good manual for using it.

I have a bunch of images, and therefore, writing it into a macro is a preferred method. For example, I have 3 images and one reference image ( All 3 images need to align to the reference image). After registration, I am more interested at the translation and rotation used for registration. For specifically, I don't expect any rotation, and therefore I want to set rotation zero. Is there any way for me to do that.

In addition,  I will appreciate if I get better ideas on how these parameters used to affect the registration, tolerance, level, stoplevel, MaterialCenterAndBBox, measure(Euclidean, MutualInformation, Threshold55, Threshold155, and Correlation).

Any good reference for this rigid registration?

Thanks!

Zhengyu

Zhengyu Pang, Ph.D.
Biochemistry and Bioanalytics Laboratory
Life Sciences and Molecular Diagnostics Technology Organization
Diagnostics, Imaging, and Biomedical Technologies
GE Global Research, K1-5B37A
One Research Circle
Niskayuna, NY 12309
T: 518-387-4015
F: 518-387-7765

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Re: Fiji ImageJ rigid registration

Ajayrama Kumaraswamy