First generation Nikon PSF

[fabrice senger-2]

Dear all,

sorry for that kind of off-topic question.

We own a Nikon TE2000 with a first generation PFS. I recently installed a
quad-band filter on that scope and realized that now I got a spot in my
images and that this spot is due to the PFS, as when I switch it of, the
spot disappears from the image.

From the documentation I found, it happens the wavelength used by the PFS
system is 770, thus if i modify my emission filter accordingly I should get
rid of the annoying signal...

Just to be sure, has someone had to face this issue as well ? What I'm not
sure about is the value 770 as this might only be the peak ...

Thanks in advance for any feedback,

Fabrice.

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Curtis Rueden-2]

Hi Fabrice,

A good place for this question would be microforum:
https://forum.microlist.org/

Regards,
Curtis

--
Curtis Rueden
Software architect, LOCI/Eliceiri lab - https://loci.wisc.edu/software
ImageJ2 lead, Fiji maintainer - https://imagej.net/User:Rueden
Have you tried the Image.sc Forum? https://forum.image.sc/



On Mon, Sep 7, 2020 at 9:29 AM Fabrice Senger <[hidden email]>
wrote:

> Dear all,
>
> sorry for that kind of off-topic question.
>
> We own a Nikon TE2000 with a first generation PFS. I recently installed a
> quad-band filter on that scope and realized that now I got a spot in my
> images and that this spot is due to the PFS, as when I switch it of, the
> spot disappears from the image.
>
> From the documentation I found, it happens the wavelength used by the PFS
> system is 770, thus if i modify my emission filter accordingly I should get
> rid of the annoying signal...
>
> Just to be sure, has someone had to face this issue as well ? What I'm not
> sure about is the value 770 as this might only be the peak ...
>
> Thanks in advance for any feedback,
>
> Fabrice.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Gary Laevsky-2]

Are you sure the PFS dichroic isn’t dirty? Happens fairly frequently to me. It’s a pain in the neck to clean.

Best,

Gary

> On Sep 7, 2020, at 10:29 AM, Fabrice Senger <[hidden email]> wrote:
>
> Dear all,
>
> sorry for that kind of off-topic question.
>
> We own a Nikon TE2000 with a first generation PFS. I recently installed a
> quad-band filter on that scope and realized that now I got a spot in my
> images and that this spot is due to the PFS, as when I switch it of, the
> spot disappears from the image.
>
> From the documentation I found, it happens the wavelength used by the PFS
> system is 770, thus if i modify my emission filter accordingly I should get
> rid of the annoying signal...
>
> Just to be sure, has someone had to face this issue as well ? What I'm not
> sure about is the value 770 as this might only be the peak ...
>
> Thanks in advance for any feedback,
>
> Fabrice.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html