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Hello. I was wondering whether someone could help me please. I am looking at the effect of growing brain cells in 3 different sera (human, serum free and fetal calf). I would like to see the effect of the sera on 8 different antibodies by flow cytometry and immunocytochemistry. The flow cytometry analysis is complete, I would now like to compare the ICC. All micrographs have been taken with a b&w camera and all with the same settings. How can I use ImageJ to quantify the fluorescence intensity? I probably wouldn't need to subtract the background as I have appropriate background ICCs. I have around 200 micrographs, so some sort of automation/plugin would be good.
Your help is very much appreciated.
Kind regards,
Sam
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