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Hi again,
there are already some topics on this, but I still don't know what options I should choose - I need to quantify the fluorescence intensity of several images with the subsequent ability to compare them. Should I use integrated density/ raw integrated density/ mean grey value? and do i need to measure the same area in each picture or can i use thresholding to get all pixel values above a certain level?
and besides this, is there a way to compensate for different exposure times?
Best,
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