Fluorescence Intensity Comparison

Previous Topic Next Topic
 
classic Classic list List threaded Threaded
1 message Options
Reply | Threaded
Open this post in threaded view
|

Fluorescence Intensity Comparison

streptopelia
Hi again,

there are already some topics on this, but I still don't know what options I should choose - I need to quantify the fluorescence intensity of several images with the subsequent ability to compare them. Should I use integrated density/ raw integrated density/ mean grey value? and do i need to measure the same area in each picture or can i use thresholding to get all pixel values above a certain level?

and besides this, is there a way to compensate for different exposure times?

Best,