On Friday 16 January 2009 15:17:03 samantha murrayI wrote:
> Hello. I was wondering whether someone could help me please. I am running
> four different experiments which I hope will correlate at the end of the
> day. The basis is the same, I am looking at the effect of growing brain
> cells (1 normal brain cell line, 1 brain tumour (high passage), and 2 brain
> tumours (low passage)) in 3 different sera (human, serum free and fetal
> calf). For two of the experiments (flow cytometry and immunocytochemistry),
> the cells are then exposed to 8 different antibodies. With Flow Cytometry,
> the geometric mean fluorescence intensity shows that there is a trend in
> both cell type and the sera in which the cells are grown. What I would like
> now is to do some stats with the ICC. I have today discovered ImageJ, but
> cannot find appropriate instructions. Unfortunately, my micrographs haven't
> been taken in a standard fashion, (number of cells in the field of view,
> exposure, brightness, contrast, etc); whereas my Flow data was all captured
> with one standard set of the instrumentation. Will it be possible to
> standardise my images in order to compare them?
No.
G.
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