Fluorescence intensity

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Fluorescence intensity

Mehdi Nikkhah
Dear all

 

I have stained the actin cytoskeleton in MDA-MB-231 cells with Alexa fluor
488 phalloidin. I intend to measure the florescence intensity of actin
cytoskeleton over the nucleus area, do I need to download a specific Plug in
for this purpose ? or can I simply do that using standard ImageJ functions ?

 

Thanks for your reply

 

Regards

Mehdi  

 

 

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Mehdi Nikkhah

PhD Candidate and Research Assistant

VT MEMS Lab

The Bradley Department of Electrical and Computer Engineering
Virginia Polytechnic Institute and State University
475 Whittemore Hall, Blacksburg, VA 24061