Fourier shape analysis

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Fourier shape analysis

Weller Andrew Francis
Dear all,

I've been attempting to play with the "Fourier descriptors" plugin by
Thomas Boudier (many thanks for that).

Unfortunately I can't get it to work. After thresholding a particle and
drawing a ROI round it with run("Create Selection"), I want to pass this
ROI to the Fourier plugin. I get the error "Selection type not supported
- the Roi must be closed."

How do I "close" the ROI? I want to use this specific ROI and not create
another for Fourier shape analysis, which the plugin seems to allow me
to do.

Thanks, Andy
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Re: Fourier shape analysis

Thomas Boudier-2
Hi Andy,

Humm I wrote it long time ago, and you need a closed shape to make the
calculation, so you should use a closed shape selection like "freehand
selection" and not "frehand line selection" that is not closed. And if you
have suggestion about the plugin, tell me.

Thomas


On Mon, 10 Jul 2006 14:12:12 +0200, Andy Weller wrote

> Dear all,
>
> I've been attempting to play with the "Fourier descriptors" plugin by
> Thomas Boudier (many thanks for that).
>
> Unfortunately I can't get it to work. After thresholding a particle and
> drawing a ROI round it with run("Create Selection"), I want to pass this
> ROI to the Fourier plugin. I get the error "Selection type not supported
> - the Roi must be closed."
>
> How do I "close" the ROI? I want to use this specific ROI and not create
> another for Fourier shape analysis, which the plugin seems to allow
> me to do.
>
> Thanks, Andy


--
Institut Curie -- Section Recherche/Research Division
http://www.curie.fr
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Re: Fourier shape analysis - how to close a selection?

Weller Andrew Francis
Thomas,

Yes this appears to be true - if I create a freehand selection, then the
Fourier descriptors are calculated correctly.

It appears that my ROI 'shape' is not closed after creating with
run("Create Selection").

How can I convert this ROI to a 'closed shape' so that the Fourier
descriptors can be calculated?

Many thanks, Andy

On Mon, 2006-07-10 at 18:40 +0300, Thomas Boudier wrote:

> Hi Andy,
>
> Humm I wrote it long time ago, and you need a closed shape to make the
> calculation, so you should use a closed shape selection like "freehand
> selection" and not "frehand line selection" that is not closed. And if you
> have suggestion about the plugin, tell me.
>
> Thomas
>
>
> On Mon, 10 Jul 2006 14:12:12 +0200, Andy Weller wrote
> > Dear all,
> >
> > I've been attempting to play with the "Fourier descriptors" plugin by
> > Thomas Boudier (many thanks for that).
> >
> > Unfortunately I can't get it to work. After thresholding a particle and
> > drawing a ROI round it with run("Create Selection"), I want to pass this
> > ROI to the Fourier plugin. I get the error "Selection type not supported
> > - the Roi must be closed."
> >
> > How do I "close" the ROI? I want to use this specific ROI and not create
> > another for Fourier shape analysis, which the plugin seems to allow
> > me to do.
> >
> > Thanks, Andy
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Re: Fourier shape analysis - how to close a selection?

Weller Andrew Francis
Dear all,

For those that are interested, I have found a solution for ROI closing
after run("Create Selection") and prior to Fourier shape analysis. In a
macro:

run("Create Selection"); // requires 1.37j
run("Enlarge...", "enlarge=0");
run("Fit Spline");
run("Fourier ", "fourier=8 output");

Cheers, Andy

On Tue, 2006-07-11 at 10:49 +0200, Andy Weller wrote:

> Thomas,
>
> Yes this appears to be true - if I create a freehand selection, then the
> Fourier descriptors are calculated correctly.
>
> It appears that my ROI 'shape' is not closed after creating with
> run("Create Selection").
>
> How can I convert this ROI to a 'closed shape' so that the Fourier
> descriptors can be calculated?
>
> Many thanks, Andy
>
> On Mon, 2006-07-10 at 18:40 +0300, Thomas Boudier wrote:
> > Hi Andy,
> >
> > Humm I wrote it long time ago, and you need a closed shape to make the
> > calculation, so you should use a closed shape selection like "freehand
> > selection" and not "frehand line selection" that is not closed. And if you
> > have suggestion about the plugin, tell me.
> >
> > Thomas
> >
> >
> > On Mon, 10 Jul 2006 14:12:12 +0200, Andy Weller wrote
> > > Dear all,
> > >
> > > I've been attempting to play with the "Fourier descriptors" plugin by
> > > Thomas Boudier (many thanks for that).
> > >
> > > Unfortunately I can't get it to work. After thresholding a particle and
> > > drawing a ROI round it with run("Create Selection"), I want to pass this
> > > ROI to the Fourier plugin. I get the error "Selection type not supported
> > > - the Roi must be closed."
> > >
> > > How do I "close" the ROI? I want to use this specific ROI and not create
> > > another for Fourier shape analysis, which the plugin seems to allow
> > > me to do.
> > >
> > > Thanks, Andy