almost all GFPs show some photoconversion by 405 nm light (also EGFP e.g.. my own observation).
for GFPuv it has been first in detail investigated by van thor, gensch et al. (2002, Nature Strucutral Biology) and later
by van thor van Thor JJ (2009) Chem Soc Rev 38:2935–2950.
But your observation is interesting, which GFP are you using (please tell us the exact mutant)?
best, thomas
Dr. Thomas Gensch
Institute of Complex Systems 4 (ICS-4), Cellular Biophysics/Institut fuer Komplexe Systeme 4, Zelluläre Biophysik
Forschungszentrum Juelich (Research Centre Juelich)
52425 Juelich
Tel:+49-2461-618068
Tel:+49-2461-614216
email:
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Von: ImageJ Interest Group [
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Betreff: GFP enhancement by 405
Please help my poor memory recall. We are noticing that when we illuminate mEOS with 405nm light to photoconvert it, we see an enhancement of the GFP signal well outside of the target ROI that persists after the 405 is turned off. I remember reading something about GFP signal enhancement bu UV light somewhere, but I cannot find it now. Does anyone know where this phenomenon is described or am I making it up? Thanks- Dave
Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
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