ImageJ to analyse lengths using intensity of bands relative to a molecular weight marker

[Beth Ashbridge]

Hi everyone,

Does anyone know how I could measure telomere length "smudges" i.e. take highest value of the histogram as the average telomere length and associate that with a molecular weight marker on the same gel?

Any ideas how I would do this using ImageJ?

thanks,



 
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[Joel Sheffield]

Could you post an image of such a gel?


On Thu, Oct 11, 2012 at 2:57 PM, Beth Ashbridge <[hidden email]> wrote:

> Hi everyone,
>
> Does anyone know how I could measure telomere length "smudges" i.e. take
> highest value of the histogram as the average telomere length and associate
> that with a molecular weight marker on the same gel?
>
> Any ideas how I would do this using ImageJ?
>
> thanks,
>
>
>
>
>      =====================================================================
>
>      Please note that this e-mail and any files transmitted from
>      Memorial Sloan-Kettering Cancer Center may be privileged,
> confidential,
>      and protected from disclosure under applicable law. If the reader of
>      this message is not the intended recipient, or an employee or agent
>      responsible for delivering this message to the intended recipient,
>      you are hereby notified that any reading, dissemination, distribution,
>      copying, or other use of this communication or any of its attachments
>      is strictly prohibited.  If you have received this communication in
>      error, please notify the sender immediately by replying to this
> message
>      and deleting this message, any attachments, and all copies and backups
>      from your computer.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs

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