Grains analysis in metals

[Laurent Barrallier]

Hi,

I am looking for macro for ImageJ to analyze grains size, shape,... in
metals. Can someone help me ?

Regards

Laurent

--
Laurent BARRALLIER

Directeur Laboratoire MécaSurf
Art et Métiers ParisTech
2, cours des Arts et Métiers
F-13617 Aix en Provence cedex 1 France

Tél. : [phone] / 14 | Fax  : [phone]
[hidden email] | http://mecasurf.aix.ensam.fr
----------------------------------------------------------

[David Hovis]

Laurent,

Generally speaking, if you can convert your images to binary (grains  
are black, grain boundaries are white), then the Analyze -> Analyze  
Particles... command will let you measure grain sizes, orientations,  
etc.  The specific measurements can be set in Analyze -> Set  
Measurements...

However, getting your typical micrograph into binary is often easier  
said than done.

--David

On Jul 8, 2008, at 4:04 PM, Laurent Barrallier wrote:

> Hi,
>
> I am looking for macro for ImageJ to analyze grains size, shape,... in
> metals. Can someone help me ?
>
> Regards
>
> Laurent
>
> --
> Laurent BARRALLIER
>
> Directeur Laboratoire MécaSurf
> Art et Métiers ParisTech
> 2, cours des Arts et Métiers
> F-13617 Aix en Provence cedex 1 France
>
> Tél. : [phone] / 14 | Fax  : [phone]
> [hidden email] | http://mecasurf.aix.ensam.fr
> ----------------------------------------------------------

[Sami Badawi-2]

Hi Laurent,

The ShapeLogic plugin contains a particle analyzer that works directly
on gray scale and color images.  This might work for you but it only
works if you have grains in a relatively uniform background, not if
you only have grains and boundaries.

It collects these particle properties:
    * Area
    * Center of gravity
    * Color
    * Std dev for color
    * Length of perimeter
    * Circularity
    * Gray value brightness
    * Bounding box
    * Number of hard corners
    * Number of inflection points
    * Number of curve arches

Link: http://www.shapelogic.org/particle.html

-Sami Badawi
http://www.shapelogic.org

[Marko Usaj]

Hello everybody!

I have following problem:

I have fluorescence images 1 with objects (intensity) 1 and fluorescence images 2 with objects (intensity) 2. But some of object are the same (fused) I want to find how many (percent) object are dual color.

Ok, one option is segmentation and then comparison/color align and then counting.

But i hear that similar procedure is to just compare pixel intensity, and perform percent calculation using number of pixels instead of object (dual color/all). Is there any plug in for do that?

Second questions is about uneven illumination. Can anyone suggest good plugin who deal with that.

Third questions is fluorescence thresholding/segment fluorescence nuclei of cells. Which is good algorithm/plugin for that?

Regardss

Marko
 

[evojcak]

For color or gray scale micrograph images I used the Heyn Method per ASTM E122.

First calibrate for the image using a micron marker or grain or object at know length by the usual Image J method.  

Then draw a horizontal line (hold down shift key)from end to end of the image. Go to Analyze then Plot Profile - this will give a plot of gray scale (converted for color images) per length across image if the Metal Grain Boundaries are black they will be closer to gray value "0" - chose "List" at bottom of window to get spreadsheet listing of length & gray scale.

Choose tool bar under EDIT for "select all" and "copy" pastes (in the Plot Profile Window) these into a Excel spreadsheet - now you can set up a logical IF function to count the number of intercept based on the gray value of a grain boundary (found by trial) - also the true length of the line will be the last length value in the listing.  

Sum the intercepts and divide by the length  - do this for several line and calculate average and standard error of the mean and find closest value in TABLE 4 in ASTM E112-10 "Average Diameter" and read across to ASTM Grain size number in first column.