Grienkiwicz equation: confused

[RajGourob Ghosh]

Dear All,

              I have to calculate the calcium conc. using the Grienkiwicz
equation. I am not able to understand the difference between the calculation
of Beta and Rmin, Rmax, as from the reports I have read I can see both are
ratios but then is it that Beta is calculated only on slide without the
sample while Rmin and Rmax are deciphered from slides loaded with sample. I
am confused in this regard . Someone please help.

[Ca ]i = KD x ß x (R -Rmin) / (Rmax- R)

 I have no idea of the difference between
 ß = fluorescence signal at 351nm at 0
 μmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 μmol.l-1 Ca2+.
 Rmin = ratio at 0 mmol.l-1 Ca2+.
 Rmax = ratio at 39.8 μmol.l-1 Ca2+.

[swaraj basu]

On Mon, 22 Dec 2008 RajGourob Ghosh wrote :
>Dear All,
>
>
 


On Mon, 22 Dec 2008 RajGourob Ghosh wrote :

>Dear All,
>
>               I have to calculate the calcium conc. using the Grienkiwicz
>equation. I am not able to understand the difference between the calculation
>of Beta and Rmin, Rmax, as from the reports I have read I can see both are
>ratios but then is it that Beta is calculated only on slide without the
>sample while Rmin and Rmax are deciphered from slides loaded with sample. I
>am confused in this regard . Someone please help.
>
>[Ca ]i = KD x ©¬ x (R -Rmin) / (Rmax- R)
>
>  I have no idea of the difference between
> ʩ = fluorescence signal at 351nm at 0
>  ¥ìmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 ¥ìmol.l-1 Ca2+.
>  Rmin = ratio at 0 mmol.l-1 Ca2+.
>  Rmax = ratio at 39.8 ¥ìmol.l-1 Ca2+.

Hello Rajgourob,
                Even I am perplexed by the same equation, I have gone through a few papers, being a novice in this area I have a faint idea how should the slides be prepared to get Rmin Rmax and Beta. With no one to help in my institute I have just hung up on the situation for the time being.
                 Swaraj

[karo03]

I am not involved with Ca concentration. However, under http://www.jbc.org/cgi/reprint/260/6/3440 
  page 3447 your problem is possibly outlined.

Regards
Karsten

Am 22.12.2008 um 07:28 schrieb RajGourob Ghosh:

> Dear All,
>
>              I have to calculate the calcium conc. using the  
> Grienkiwicz
> equation. I am not able to understand the difference between the  
> calculation
> of Beta and Rmin, Rmax, as from the reports I have read I can see  
> both are
> ratios but then is it that Beta is calculated only on slide without  
> the
> sample while Rmin and Rmax are deciphered from slides loaded with  
> sample. I
> am confused in this regard . Someone please help.
>
> [Ca ]i = KD x ß x (R -Rmin) / (Rmax- R)
>
> I have no idea of the difference between
> ß = fluorescence signal at 351nm at 0
> μmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 μmol.l-1 Ca2+.
> Rmin = ratio at 0 mmol.l-1 Ca2+.
> Rmax = ratio at 39.8 μmol.l-1 Ca2+.

[RajGourob Ghosh]

Thank you Karsten for the link.

On Tue, Dec 23, 2008 at 6:16 PM, Karsten Rodenacker <[hidden email]>wrote:

> I am not involved with Ca concentration. However, under
> http://www.jbc.org/cgi/reprint/260/6/3440 page 3447 your problem is
> possibly outlined.
>
> Regards
> Karsten
>
> Am 22.12.2008 um 07:28 schrieb RajGourob Ghosh:
>
>
>  Dear All,
>>
>>             I have to calculate the calcium conc. using the Grienkiwicz
>> equation. I am not able to understand the difference between the
>> calculation
>> of Beta and Rmin, Rmax, as from the reports I have read I can see both are
>> ratios but then is it that Beta is calculated only on slide without the
>> sample while Rmin and Rmax are deciphered from slides loaded with sample.
>> I
>> am confused in this regard . Someone please help.
>>
>> [Ca ]i = KD x ß x (R -Rmin) / (Rmax- R)
>>
>> I have no idea of the difference between
>> ß = fluorescence signal at 351nm at 0
>> μmol.l-1 Ca2+ / fluorescence signal at 364 nm at 39.8 μmol.l-1 Ca2+.
>> Rmin = ratio at 0 mmol.l-1 Ca2+.
>> Rmax = ratio at 39.8 μmol.l-1 Ca2+.
>>
>