How to count only bubbles that are in focus

[jmcginnity]

Hi, I'm trying to get size distribution information from photos of bubbles in water. However I only want to measure those bubbles that are in focus. Can anyone assist with this. An image is attached. Thanks for any help, Justin

[Gabriel Lapointe-4]

Hi Justin,
I would try a variance filter (increases intensity at sharp edges, in your
case the bubbles in focus). Then I would do simple intensity threshold.

Good luck,
*Gabriel Lapointe, M.Sc.*
Lab Manager / Microscopy Specialist
Concordia University, Biology Department
7141 Sherbrooke St. West SP 534
Montréal QC H4B 1R6 Canada
Lab : [phone] x5988
Office : [phone] x3008
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http://gabriellapointe.ca



2012/11/27 jmcginnity <[hidden email]>

> Hi,I'm trying to get size distribution information from photos of bubbles
> in
> water.  However I only want to measure those bubbles that are in focus.
>  Can
> anyone assist with this. An image is attached.
> <http://imagej.1557.n6.nabble.com/file/n5000970/Bubble_image.jpg> Thanks
> for
> any help,Justin
>
>
>
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> Sent from the ImageJ mailing list archive at Nabble.com.
>
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