Embryonic cells shape detection and area computation help

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Embryonic cells shape detection and area computation help

panovr
Hi,
  I have some embryonic cells images, and I want to detect the individual
cell's shape and compute their area, and count.
  May others who with medical image processing and embryonic cell image
processing experience help me, and give me some guidance?
  For your convenience, I have prepared some embryonic cells test images,
the download URL is:
https://docs.google.com/open?id=0BwWaVCRVWuFdSkw5a1MtaWRhM1E
  Thanks!

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Yili Zhao

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Re: Embryonic cells shape detection and area computation help

Adam Hughes
How many cells per image?  ImageJ's analyze particles builtin methods will
count and record the area of paritcles ona  picture; however, if there are
only a few cells per image, you may want to just set the length scale and
do some measurements by hand using the meausre tool

On Sun, Nov 25, 2012 at 6:54 AM, Yili Zhao <[hidden email]> wrote:

> Hi,
>   I have some embryonic cells images, and I want to detect the individual
> cell's shape and compute their area, and count.
>   May others who with medical image processing and embryonic cell image
> processing experience help me, and give me some guidance?
>   For your convenience, I have prepared some embryonic cells test images,
> the download URL is:
> https://docs.google.com/open?id=0BwWaVCRVWuFdSkw5a1MtaWRhM1E
>   Thanks!
>
> --
> Yili Zhao
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Embryonic cells shape detection and area computation help

carlo bianco
hello
I think that manual counting is more suitable  and efficient,
also because cells are clustered.
Analyze particles works very well for cells/elements dispersed on a background or
"empty" parenchyma (testes tubules inverted, placental villi).
In my opinion analyze particles could be useful to count embryos on
a petri dish.

I hope these opinion are useful.
Good luck
Carlo Bianco
Bologna.Italy









________________________________
 Da: Adam Hughes <[hidden email]>
A: [hidden email]
Inviato: Domenica 25 Novembre 2012 21:13
Oggetto: Re: Embryonic cells shape detection and area computation help
 
How many cells per image?  ImageJ's analyze particles builtin methods will
count and record the area of paritcles ona  picture; however, if there are
only a few cells per image, you may want to just set the length scale and
do some measurements by hand using the meausre tool

On Sun, Nov 25, 2012 at 6:54 AM, Yili Zhao <[hidden email]> wrote:

> Hi,
>   I have some embryonic cells images, and I want to detect the individual
> cell's shape and compute their area, and count.
>   May others who with medical image processing and embryonic cell image
> processing experience help me, and give me some guidance?
>   For your convenience, I have prepared some embryonic cells test images,
> the download URL is:
> https://docs.google.com/open?id=0BwWaVCRVWuFdSkw5a1MtaWRhM1E
>   Thanks!
>
> --
> Yili Zhao
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Re: Embryonic cells shape detection and area computation help

panovr
In reply to this post by panovr
Hi Adam,
  maybe your can give an eye at some embryonic cells test images:

https://docs.google.com/open?id=0BwWaVCRVWuFdSkw5a1MtaWRhM1E

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Re: Embryonic cells shape detection and area computation help

panovr
In reply to this post by panovr
Hi Carlo,
  thanks for your opinions, and I want to do the measure and count automatically, maybe by a custom plugin or by a custom script.
  Thanks!

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