How to crop two images

[Chunfang Zhang]

Hi,

I am a new user of Image J.

Just wondering if there is an easy way to crop two images (two fluorescent
Western images from the same membrane) to the same size and area. Thank you.

Kind regards

Chunfang

[Xulong Wang]

Sounds doable, please provide a picture, and specify your question more detailedly.

---
Xulong Wang
Neuroscience Postdoc in UCSD





On Aug 4, 2011, at 11:42 PM, Chunfang Zhang wrote:

> Hi,
>
> I am a new user of Image J.
>
> Just wondering if there is an easy way to crop two images (two fluorescent
> Western images from the same membrane) to the same size and area. Thank you.
>
> Kind regards
>
> Chunfang

[Gabriel Landini]

On Friday 05 Aug 2011, Chunfang Zhang wrote:
> Just wondering if there is an easy way to crop two images (two fluorescent
> Western images from the same membrane) to the same size and area. Thank
> you.

At least 4 ways:
Convert the 2 images to a stack, crop the stack, then convert the stack to
images.
Or find the "Sync Windows" plugin to create the same ROI in both images and
crop each one
Or create a ROI in the first image, then click one the second image and use
Restore Selection to transfer the ROI from the 1st image.
Or specify the ROI in a short macro with makeRectangle(x, y, width, height),
crop and run the same macro on the 2nd image.

Cheers

G

[Antoinette]

Did you mean that you want to crop two or more images at the same time? I didn't use such batch image cropping tool, each time I need to crop images, I have to deal with the image one by one.