How to define the slice interval for an image stack

[Sumit Nanda]

Hello,

I have some image stacks with 2 um slice interval. With 6 images in the
stack, the total Z-thickness should be 12 um. How do I define the
interslice interval within the image J, before saving it 3D tiff? Also,
when I load a CZI file, how do check if the interslice interval is properly
set at 2 um?

Thanks in Advance.
Sumit

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[Michael Schmid]

On Sat, March 7, 2015 00:26, Sumit Nanda wrote:
> I have some image stacks with 2 um slice interval. With 6 images in the
> stack, the total Z-thickness should be 12 um. How do I define the
> interslice interval within the image J, before saving it 3D tiff? Also,
> when I load a CZI file, how do check if the interslice interval is
> properly
> set at 2 um?

Hi Sumit,

You can examine and change the voxel depth (= slice spacing) in
Image>Properties.
In a macro, use
  getVoxelSize(width, height, depth, unit)
to read the voxel depth.

Michael

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