How to measure voxels from 3D images

[Cobus]

Hello

I created 3D images from 2D pictures using the Interactive 3D surface plot (V2.32) plugin. These images are based on the colour intensity if i understand correct. What I would like to do is to measure the volume created in the 3D image. It is an irregular shape and I would need to measure the voxels in the specific ROI of these images.

I have looked at quite a few plugins without any luck. A lot of programs can create the 3D image but there is no quantitative out put which I know of. I know this must be possible, but I just can't the right program. I also unfortunately have no programming background. Any help would be appreciated.

Best regards
Cobus

[Michael Schmid]

Hi Cobus,

the volume in the 3D image is simply the integral of the 2D image,  
i.e., the sum of all values inside the roi. Use Analyze>Measure on  
the 2D image and multiply the Mean by the Area to get it.

Michael
________________________________________________________________

On 21 Jul 2011, at 15:07, Cobus wrote:

> Hello
>
> I created 3D images from 2D pictures using the Interactive 3D  
> surface plot
> (V2.32) plugin. These images are based on the colour intensity if i
> understand correct. What I would like to do is to measure the  
> volume created
> in the 3D image. It is an irregular shape and I would need to  
> measure the
> voxels in the specific ROI of these images.
>
> I have looked at quite a few plugins without any luck. A lot of  
> programs can
> create the 3D image but there is no quantitative out put which I  
> know of. I
> know this must be possible, but I just can't the right program. I also
> unfortunately have no programming background. Any help would be  
> appreciated.
>
> Best regards
> Cobus

[Cobus]

Thank you for your comments. My question arose from the loss of intensity when calculating area using pixels of a given ROI. This is the traditional way of processing the data I am currently generating. I have seen that in some cases the area and intensity of my ROI's do not necessarily correlate. To address this issue I need to consider intensity. Do you know if there is a way to get the sum of the voxels of a ROI instead of the mean? If this is possible can you get the colour (code) and the amount of pixels for each individual colour?