How to save image with overlay.

[Adam Hacking]

Hi,
I'm generating images using Analyze Particles. I would to save the resulting graphical overlay of the results on the image with the image. Is there some way to do this, like flattening an image in Photoshop ?

Thx Adam




     

[Colin Poczatek]

Wayne added what I think you're looking for in ij 1.42f, ability to grab
an RGB version of the current image with whatever is drawn on it...

https://list.nih.gov/cgi-bin/wa?A2=ind0812&L=IMAGEJ&D=0&I=-3&P=22542

Adam Hacking wrote:

> Hi,
> I'm generating images using Analyze Particles. I would to save the resulting graphical overlay of the results on the image with the image. Is there some way to do this, like flattening an image in Photoshop ?
>
> Thx Adam
>
>
>
>
>      
>  

The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
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[Joel Sheffield]

Adam,

You can do this in several steps --It's a bit kludgy, but it will
work.

Once you have set your thresholds and obtained the Analyze Particles
image,

1.  Remove the threshold color from your original 8-bit image by
selecting the grays lut.

2.  Convert the outline image into a gray scale by selecting that
same lut.

3.  If you want the outlines to be white, invert the image
(Edit>invert)  (this does not turn the image upside down, it reverses
the lut)  If you want the outines to be black, do nothing.

4.  Select the outline image.  I generally use ctl-A,
5.  Copy the outline image.  I generally use ctl-C
6.  Paste the outline image on your original.

Horrors! --it seems to have simply covered the original.  Now, in the
Edit menu, there is an item called "paste control".  If you click on
it, you will see many options.  You can "blend" the two images --
rather like photoshop-  or you can make either black or white
transparent.  Play around with these and see what you like.

Then, you can save the resulting image.

Best of luck

Joel


Date sent:       Mon, 8 Jun 2009 17:43:19 -0400
Send reply to:   ImageJ Interest Group <[hidden email]>
From:           Colin Poczatek <[hidden email]>
Subject:         Re: How to save image with overlay.
To:             [hidden email]

> Wayne added what I think you're looking for in ij 1.42f, ability to grab
> an RGB version of the current image with whatever is drawn on it...
>
> https://list.nih.gov/cgi-bin/wa?A2=ind0812&L=IMAGEJ&D=0&I=-3&P=22542
>
> Adam Hacking wrote:
> > Hi,
> > I'm generating images using Analyze Particles. I would to save the resulting graphical overlay of the results on the image with the image. Is there some way to do this, like flattening an image in Photoshop ?
> >
> > Thx Adam
> >
> >
> >
> >
> >      
> >  
>
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs