I need advice to find out the total surface occupied by black stains of some images

[Ion Andronache]

[Andrei Stefan]

Hi/Servus,

I did a diagram of what I think you should do to achieve your goal.
I hope it helps!


Best regards,
Andrei

2015-04-03 18:34 GMT+01:00 Ion C. Andronache <[hidden email]>:

>


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Andrei Cătălin ȘTEFAN, MRCVS, DVM, MVSc

E-mail: [hidden email]

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[Ion Andronache]

[Vergara, Leoncio A.-2]

Can you use “limit to threshold” ?

On 4/3/15, 1:13 PM, "Andrei Catalin Stefan" <[hidden email]>
wrote:

>Hi/Servus,
>
>I did a diagram of what I think you should do to achieve your goal.
>I hope it helps!
>
>
>Best regards,
>Andrei
>
>2015-04-03 18:34 GMT+01:00 Ion C. Andronache <[hidden email]>:
>
>>
>
>
>--
>
>Andrei Cătălin ȘTEFAN, MRCVS, DVM, MVSc
>
>E-mail: [hidden email]
>
>--
>ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Herbie-4]

Ion,

after setting the proper scale, do the following:

1.
 From "Image > Lookup Tables" choose "Invert LUT".
2.
 From "Image > Adjust > Threshold..." set the threshold somewhere
between 0 and 255. (My dialog opens with a suitable choice.) Then click
"Apply".
3.
 From "Analyze > Set Measurement" check "Area" and "Limit to threshold".
4.
 From "Analyze" choose "Measure".

The value I get is:
image2.tif 4807.915

HTH

Herbie

::::::::::::::::::::::::::::::::::::::::::::::
Am 03.04.15 um 20:56 schrieb Ion C. Andronache:
> Thank you very much, but it is a problem. I want to know area only
> for black pixels. 30538.92734 is for all pixels. It is a possibility
> in Imagej?
>
> -- ImageJ mailing list:http://imagej.nih.gov/ij/list.html
>

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[Jacqueline Ross]

Hi Ion,

One further point. I think you need to measure the total area occupied by the tissue as well since you have a lot of white space around the section and if you are measuring multiple sections, they may be different in size, which will affect your comparisons. Area fraction may be worth considering.

Kind regards,

Jacqui


Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Ion C. Andronache
Sent: Saturday, 4 April 2015 7:57 a.m.
To: [hidden email]
Subject: Re: I need advice to find out the total surface occupied by black stains of some images



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