Image J recurrent crash

[Laura Lucia Prieto Godino]

Dear List,

I am opening around 6 confocal stacks at a time (which normally it is  
not a problem), then in one of them I am using substract background,  
and then gaussian filter, and hten I am adjusting the birghtness and  
contrast. Then I am color-merging this stack with another one, and  
then if I am lucky and it does not crash I make a z-projection of  
this, but most of the times ImageJ keeps crashing before I can save  
the results. Does anybody have an idea how can I solve this?I am using  
a Mac version 10.5.8. And as I am sure that it will crash again I can  
also copy and send you the crash report generated by mac (if that helps)
thanks
Lucia


  Lucia Prieto Godino
PhD student.
Department of Zoology,
Downing street
University of Cambridge.
UK
http://www.neuroscience.cam.ac.uk/directory/profile.php?lauralucia

[Teresa W]

 I reckon poor IJ gets a bit confused sometimes w so many processes.

Altho I have not tested in any scientific manner, this may help. Basically I save save interim calculation stacks as generic name stack.tiffs e.g. result-1.tif  Mask-2.tif on the desktop, and make sure I close what I don't need to work on immediately. I open the required interim stack files within the macro sequence at th stage I need to use them again, not forgetting to keep tidy and close as I go.

This seemed to reduce my crash rate when running a string of manipulations on stacks from a recorded macro. No need to create a new file for each new confocal stack, just rewrite over the generic stack.tif name.