ImageGauge/MultiGauge Software / BAS Image File Analysis

[ccoffman]

Does anyone have experiance with replicating the functionality of the (now
dead) ImageGauge/MultiGauge software package that was used with FUJI
imaging products?

The Fugi imaging system (such as their phosphorimager) outputs .img files
and an accompanying .inf file.  The .inf file is just text and contains
image parameters and the .img file is the image and can be imported into
imagej as a RAW file (if you read the inf file and can pull out the image
dimensions and specify that it is a 16-bit unsigned image).

This gives you the image but there is no plugin or otherwise elegant way to
do the kinds of quantification and analysis you can do with the multigauge
software (which you can't even get anymore unless you know someone who will
give you an copy, and that's technically against the EULA (so much for
proprietary closed source software)).

I was just wondering since this so called "BAS image file"  format is so
common if someone has developed alternative ways of working with it.

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[Neil Fazel]

Hi Clayton,

    Have you checked the ISAC plugin?

http://rsb.info.nih.gov/ij/plugins/isac.html

Neil

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[ccoffman]

Very nice, thanks!

This makes opening the files easier, but I still am wondering how I could
do quantification and get similar result to the MultiGauge package.  I
probably should spend some more time working with it.


On Wed, Feb 19, 2014 at 12:57 PM, Neil Fazel <[hidden email]>wrote:

> Hi Clayton,
>
>     Have you checked the ISAC plugin?
>
> http://rsb.info.nih.gov/ij/plugins/isac.html
>
> Neil
>
>

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[Neil Fazel]

Once you've used the plugin to open the Fuji scanner's logarithmic output (known as QL or quantum levels), you can write a simple ImageJ macro to convert each pixel value to PSL (photostimulated luminescence); there may even be a plugin for that. Then on the resulting image you can do your analysis using ImageJ capabilities.

My experience, after having used Mathematica and other tools, is that Imagej is the natural environment for analysis of imaging plate scanner output. You can do more with ImageJ than any Fuji-provided quantitative analysis tool.

Neil

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[ccoffman]

interesting, I will mess with it some more.

thanks a lot, this is very helpful!


On Wed, Feb 19, 2014 at 1:26 PM, Neil Fazel <[hidden email]>wrote:

> Once you've used the plugin to open the Fuji scanner's logarithmic output
> (known as QL or quantum levels), you can write a simple ImageJ macro to
> convert each pixel value to PSL (photostimulated luminescence); there may
> even be a plugin for that. Then on the resulting image you can do your
> analysis using ImageJ capabilities.
>
> My experience, after having used Mathematica and other tools, is that
> Imagej is the natural environment for analysis of imaging plate scanner
> output. You can do more with ImageJ than any Fuji-provided quantitative
> analysis tool.
>
> Neil
>
>
>

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[cfhaugaard]

Would like to do something similar. Did you end up on a nice solution??