ImageJ LSM to Tif files

[CB Mahoney]

Hello
 
I would like to convert my lsm files (2channels and z-stack) to multilayer tiff files to be used in Autoquant Autodeblur v 9.2.  Does anyone know what plugin to use in image J or the steps to do so.
 
Thanks so much for your time
 
Carrie

[Graham Wright]

Carey,

I thought that that AutoQuant software could open the Zeiss .lsm files
directly?

If not, then you can use the LOCI plugins (
http://www.loci.wisc.edu/ome/formats-imagej.html) to open the files in
ImageJ and then save them into any of the ImageJ formats in the usual way.

I hope this helps,
Graham


2009/8/20 CB Mahoney <[hidden email]>

> Hello
>
> I would like to convert my lsm files (2channels and z-stack) to multilayer
> tiff files to be used in Autoquant Autodeblur v 9.2.  Does anyone know what
> plugin to use in image J or the steps to do so.
>
> Thanks so much for your time
>
> Carrie
>

[nmilyaev]

Not sure about that special TIFF format, to obtain simple set of TIFFs out of it, use
 File -> Save As -> Image Sequence; specify output folder and you are done.

Hope that helps