ImageJ Related Question About Software

[ev34]

Hi,

Could anyone recommend a good, (preferrably freeware) software to
capture microscope images? I am mostly interested in capturing
fluorescence in my images, using a bandwith filter in the low to mid 500
nm. The microscope I use is the Nikon inverted Eclipse TE2000-S, the
camera is a relatively old, automatic Cool Snap brand, so I need to be
sure that the software is compatible with both of these. I have been
using the Roper Scientific software and have been having quite a few
problems with the settings, especially with getting a sharp, focused
image (sometimes I get a completely black screen showing up without any
image for no apparent reason). Because I do not have a lot of
fluorescence in my specimens, I need something that could be configured
to be quite sensitive. I use ImageJ to analyze the fluorescence.

Thanks!

Lena

Hi:

We have had some success with RSImage.  Its an image capture program made by
roperscientific - It may work if your camera uses the pvcam interface
roperscientfic uses.  I

http://www.photomet.com/software_rsimage.html

Another option is micromanager http://www.micro-manager.org/index.php

It an iimage capture program designed to work with ImageJ - I've only done
preliminary testing with micro-managre so I can't comment on it.  I really
like  the idea behind micro-mananger - an opensource system that will work
with as many different systems as possible.

Gord


On Wednesday 13 December 2006 2:22 pm, Lena Vayndorf wrote:

> Hi,
>
> Could anyone recommend a good, (preferrably freeware) software to
> capture microscope images? I am mostly interested in capturing
> fluorescence in my images, using a bandwith filter in the low to mid 500
> nm. The microscope I use is the Nikon inverted Eclipse TE2000-S, the
> camera is a relatively old, automatic Cool Snap brand, so I need to be
> sure that the software is compatible with both of these. I have been
> using the Roper Scientific software and have been having quite a few
> problems with the settings, especially with getting a sharp, focused
> image (sometimes I get a completely black screen showing up without any
> image for no apparent reason). Because I do not have a lot of
> fluorescence in my specimens, I need something that could be configured
> to be quite sensitive. I use ImageJ to analyze the fluorescence.
>
> Thanks!
>
> Lena

--
Gordon J. Holtslander Dept. of Biology
[hidden email] University of Saskatchewan
Tel [phone] Science Place
Fax [phone] Saskatoon SK., CANADA
homepage.usask.ca~gjh289 S7N 5E2

[Nico Stuurman-4]

Micro-Manager supports the Nikon TE2000 and PVCAM-compatible  
cameras.  So, if your old CoolSnap can be driven with the PVCAM  
library (http://www.photomet.com/software_pvcam.html) there is a fair  
chance all will work with Micro-Manager (http://micro-manager.org).  
We have so far only tested the software with the Nikon TE2000E, so we  
are quite interested in your experience with the TE2000-S.  You can  
post follow-up questions/remarks either here or on the Micro-Manager  
list (https://lists.sourceforge.net/lists/listinfo/micro-manager- 
general).

Best,

Nico

On Dec 14, 2006, at 3:03 PM, Gordon J. Holtslander wrote:

> Hi:
>
> We have had some success with RSImage.  Its an image capture  
> program made by
> roperscientific - It may work if your camera uses the pvcam interface
> roperscientfic uses.  I
>
> http://www.photomet.com/software_rsimage.html
>
> Another option is micromanager http://www.micro-manager.org/index.php
>
> It an iimage capture program designed to work with ImageJ - I've  
> only done
> preliminary testing with micro-managre so I can't comment on it.  I  
> really
> like  the idea behind micro-mananger - an opensource system that  
> will work
> with as many different systems as possible.
>
> Gord
>
>
> On Wednesday 13 December 2006 2:22 pm, Lena Vayndorf wrote:
>> Hi,
>>
>> Could anyone recommend a good, (preferrably freeware) software to
>> capture microscope images? I am mostly interested in capturing
>> fluorescence in my images, using a bandwith filter in the low to  
>> mid 500
>> nm. The microscope I use is the Nikon inverted Eclipse TE2000-S, the
>> camera is a relatively old, automatic Cool Snap brand, so I need  
>> to be
>> sure that the software is compatible with both of these. I have been
>> using the Roper Scientific software and have been having quite a few
>> problems with the settings, especially with getting a sharp, focused
>> image (sometimes I get a completely black screen showing up  
>> without any
>> image for no apparent reason). Because I do not have a lot of
>> fluorescence in my specimens, I need something that could be  
>> configured
>> to be quite sensitive. I use ImageJ to analyze the fluorescence.
>>
>> Thanks!
>>
>> Lena
>
> --
> Gordon J. Holtslander Dept. of Biology
> [hidden email] University of Saskatchewan
> Tel [phone] Science Place
> Fax [phone] Saskatoon SK., CANADA
> homepage.usask.ca~gjh289 S7N 5E2