Images Strategy

[Houssam hajjoul]

Dear ImageJusers,

I wanna Track some particles(like beads).I have a problem of photobleaching and noise.
I found some method to reward photobleaching such as described in :

http://www.uhnresearch.ca/facilities/wcif/imagej/t.htm#t_bleach

But they wrote that
/"Since bleaching is often not mono-exponential, quantification of fluorescence intensities after bleach correction is not possible. This plugin should really only be used to enhance time-course movies for presentation rather than quantification".

/The question is :
if I apply some image processing like this plugin or like enhance contrast such backround correction or any other filters" did I lose in quantification of my particles?
 or I can do whatever I wanna of image processing, then I can use my plugin of particles tracker?*
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Thanks in advance.
Best regards.
Houssam

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Best Regards
Houssam Hajjoul
Phd student
Groupe N2IS
tel:[phone]
LAAS-CNRS Toulouse