Immunohistochemistry staining

[Caterina Conti]

Hi everybody,

I am new on ImageJ.
I have to analyse the total area of the epithelium of some airways treated with immunohistochemistry for a protein,stained with haematoxylin,and then do a percentage of the areas stained with DAB.
I have to analyse only the staining in the epithelium trying to eliminate the one in the lumen of the airway (it is a mucin immunohistochemistry staining and it's not specific only for the epithelium).
I wanted to know if there was a specific way to do that.
Thank you

Caterina

Inviato da iPhone

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[carlo bianco]

hi
you can try color segmentation plugin

http://bigwww.epfl.ch/sage/soft/colorsegmentation/
good luck




Il Mercoledì 26 Febbraio 2014 15:33, Caterina Conti <[hidden email]> ha scritto:
 
Hi everybody,

I am new on ImageJ.
I have to analyse the total area of the epithelium of some airways treated with immunohistochemistry for a protein,stained with haematoxylin,and then do a percentage of the areas stained with DAB.
I have to analyse only the staining in the epithelium trying to eliminate the one in the lumen of the airway (it is a mucin immunohistochemistry staining and it's not specific only for the epithelium).
I wanted to know if there was a specific way to do that.
Thank you

Caterina

Inviato da iPhone

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html