Import image sequence convert to single channel

[Holloway, Michael]

Importing my tiffs gives a stack with empty red and blue images.  Selecting
"convert to 8-bit" doesn't seem to do anything, and I can't locate a
conversion utility.  I'm guessing I need something that will convert my RGB
tiffs to a single channel.  Do I do this in batch before importing the
sequence, or is there a way of fixing the stack?

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[Aryeh Weiss]

On 9/16/13 6:32 AM, Holloway, Michael wrote:
> Importing my tiffs gives a stack with empty red and blue images.  Selecting
> "convert to 8-bit" doesn't seem to do anything, and I can't locate a
> conversion utility.  I'm guessing I need something that will convert my RGB
> tiffs to a single channel.  Do I do this in batch before importing the
> sequence, or is there a way of fixing the stack?
>
>
If it is a stack of RGB images, you can use image>color>split channels ,
then close the two empty channels.
If you have composite images, you can also split channels and then close
the empty channels.

--aryeh

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Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

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[Holloway, Michael]

Thanks for the responses, but I errored in describing my problem.  I have a
series of slices with only green label unfortunately saved as RGB.
 Importing gives what you would expect, black images for red and blue
interspersed with green.  Channel selection doesn't seem to work with an
imported stack.  Just get back a message that I need to open a multichannel
image.  What I need to know is if there's a batch method of converting my
slice images prior to importing, or if there's a function to split the
channels of the stack.  Can't find instruction on doing this in import
directions, and can't find discussion of this elsewhere.

Thanks,
Mike Holloway


On Mon, Sep 16, 2013 at 2:34 AM, Aryeh Weiss <[hidden email]> wrote:

> On 9/16/13 6:32 AM, Holloway, Michael wrote:
>
>> Importing my tiffs gives a stack with empty red and blue images.
>>  Selecting
>> "convert to 8-bit" doesn't seem to do anything, and I can't locate a
>> conversion utility.  I'm guessing I need something that will convert my
>> RGB
>> tiffs to a single channel.  Do I do this in batch before importing the
>> sequence, or is there a way of fixing the stack?
>>
>>
>>  If it is a stack of RGB images, you can use image>color>split channels ,
> then close the two empty channels.
> If you have composite images, you can also split channels and then close
> the empty channels.
>
> --aryeh
>
> --
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph:  972-3-5317638
> FAX: [phone]
>
>

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

[Holloway, Michael]

Sorry again.  I quickly discovered batch converter.java and problem solved.
 But it does seem that the "convert to 8-bit" option in the import> image
sequence dialog is supposed to handle the RGB to single channel conversion.
 It doesn't.


On Mon, Sep 16, 2013 at 8:56 AM, Holloway, Michael <
[hidden email]> wrote:

> Thanks for the responses, but I errored in describing my problem.  I have
> a series of slices with only green label unfortunately saved as RGB.
>  Importing gives what you would expect, black images for red and blue
> interspersed with green.  Channel selection doesn't seem to work with an
> imported stack.  Just get back a message that I need to open a multichannel
> image.  What I need to know is if there's a batch method of converting my
> slice images prior to importing, or if there's a function to split the
> channels of the stack.  Can't find instruction on doing this in import
> directions, and can't find discussion of this elsewhere.
>
> Thanks,
> Mike Holloway
>
>
> On Mon, Sep 16, 2013 at 2:34 AM, Aryeh Weiss <[hidden email]> wrote:
>
>> On 9/16/13 6:32 AM, Holloway, Michael wrote:
>>
>>> Importing my tiffs gives a stack with empty red and blue images.
>>>  Selecting
>>> "convert to 8-bit" doesn't seem to do anything, and I can't locate a
>>> conversion utility.  I'm guessing I need something that will convert my
>>> RGB
>>> tiffs to a single channel.  Do I do this in batch before importing the
>>> sequence, or is there a way of fixing the stack?
>>>
>>>
>>>  If it is a stack of RGB images, you can use image>color>split channels
>> , then close the two empty channels.
>> If you have composite images, you can also split channels and then close
>> the empty channels.
>>
>> --aryeh
>>
>> --
>> Aryeh Weiss
>> Faculty of Engineering
>> Bar Ilan University
>> Ramat Gan 52900 Israel
>>
>> Ph:  972-3-5317638
>> FAX: [phone]
>>
>>
>

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