Dear ImageJ colleagues,
I am working on ImageJ 1.46r version and noticed that sometimes I could not set threshold manually. A image mask is generated using 16-bit image, so that cells are numbered from 1 to 4000, and the background is set at 0. I would like to identify cell number 1. So that the best way for me to do it is to manually threshold to low 1 and high 2. However, the ImageJ threshold/set does not seem to like it, and it gives me the background highlighted too. Is this a known issue, and how do I avoid this.
Thanks,
Zhengyu
Zhengyu Pang, Ph.D.
Biochemistry and Bioanalytics Laboratory
Life Sciences and Molecular Diagnostics Technology Organization
Diagnostics and Biomedical Technologies
GE Global Research, K1-5B37A
One Research Circle
Niskayuna, NY 12309
T: 518-387-4015
F: 518-387-7765
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