Intensity analysis for ROS imaging

[Sougat Misra]

Hi Dear,

I did some confocal microscopy for ROS (Reactive oxygen species) imaging in
hepatocytes using Zeiss LSM 410. I want to measure the fluorescence intensity
from each cell. However, I don't know how to do it using ImajeJ. If anyone can
help me in this regard, it will be great.

Since I am a new user, I apologize for asking such a simple question. However,
your help will be highly appreciated.

Thanking you,

Sougat Misra
Ph.D. Candidate
University of Saskatchewan

[daschneider9]

Sougat,

"Me, too". I, too, am a relatively new user that's not a programmer but very interested in doing a correct/legitimate job at fluorescence densitometry in tissue slice. I look forward to seeing what advice the experts here can give.

Thanks for your question.
Dave

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Sougat Misra
Sent: Friday, January 29, 2010 8:28 AM
To: [hidden email]
Subject: Intensity analysis for ROS imaging

Hi Dear,

I did some confocal microscopy for ROS (Reactive oxygen species) imaging in
hepatocytes using Zeiss LSM 410. I want to measure the fluorescence intensity
from each cell. However, I don't know how to do it using ImajeJ. If anyone can
help me in this regard, it will be great.

Since I am a new user, I apologize for asking such a simple question. However,
your help will be highly appreciated.

Thanking you,

Sougat Misra
Ph.D. Candidate
University of Saskatchewan

[John Oreopoulos]

Sougat, a good starting place is the MacBiophotonics web pages  
maintained by Tony Collins:

http://www.macbiophotonics.ca/imagej/
http://www.macbiophotonics.ca/imagej/intensity_vs_time_ana.htm

There are many good tutorials on how to do basic image analysis with  
ImageJ.

Cheers


John Oreopoulos, BSc,
PhD Candidate
University of Toronto
Institute For Biomaterials and Biomedical Engineering
Centre For Studies in Molecular Imaging

On 29-Jan-10, at 11:27 AM, Sougat Misra wrote:

> Hi Dear,
>
> I did some confocal microscopy for ROS (Reactive oxygen species)  
> imaging in
> hepatocytes using Zeiss LSM 410. I want to measure the fluorescence  
> intensity
> from each cell. However, I don't know how to do it using ImajeJ. If  
> anyone can
> help me in this regard, it will be great.
>
> Since I am a new user, I apologize for asking such a simple  
> question. However,
> your help will be highly appreciated.
>
> Thanking you,
>
> Sougat Misra
> Ph.D. Candidate
> University of Saskatchewan

[Li-Ping Yuan]

At a very basic level, mous
Need more descriptions of what you want?

At a very basic level, mouse over cell pixels and read the intensity from the menu bar.  You probably want more...  an intensity profile along a straight line?  an intensity histogram (and statistics) of a ROI?  or some more complicated?
 Li-Ping (Li) Yuan





________________________________
From: "Schneider, David A." <[hidden email]>
To: [hidden email]
Sent: Fri, January 29, 2010 10:45:17 AM
Subject: Re: Intensity analysis for ROS imaging

Sougat,

"Me, too". I, too, am a relatively new user that's not a programmer but very interested in doing a correct/legitimate job at fluorescence densitometry in tissue slice. I look forward to seeing what advice the experts here can give.

Thanks for your question.
Dave

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Sougat Misra
Sent: Friday, January 29, 2010 8:28 AM
To: [hidden email]
Subject: Intensity analysis for ROS imaging

Hi Dear,

I did some confocal microscopy for ROS (Reactive oxygen species) imaging in
hepatocytes using Zeiss LSM 410. I want to measure the fluorescence intensity
from each cell. However, I don't know how to do it using ImajeJ. If anyone can
help me in this regard, it will be great.

Since I am a new user, I apologize for asking such a simple question. However,
your help will be highly appreciated.

Thanking you,

Sougat Misra
Ph.D. Candidate
University of Saskatchewan