Kalman Stack Filter not working

[JellePostma]

Dear all,

I am trying to use FIJI to run the Kalman Stack Filter on a TIRF time
series.

I run FIJI (ImageJ 1.52i) and Java 1.8.0_66 (Win10/64 bit)

The time series is a *.czi file acquired using the Zeiss Elyra:

https://i.imgur.com/Szeoct7.jpg

Instead of running on the already opened stack, the Kalman Stack Filter asks
for a file location.

Selecting my TIRF time series opens the first dialog screen succesfully:

https://i.imgur.com/NoekAYf.png

I then proceed without changing anything, and the next two screens open:

https://i.imgur.com/HDfUQhU.png

Upon clicking "OK" the Bio-Formats importer opens:

https://i.imgur.com/yKOgBoI.png

And I continue without changing anything, and the CZI file opens.

However, I immediately get another Bio-Formats importer screen. I only
wanted to import one time series!

When I click on OK, it opens another instance of the CZI image, and then
throws an Exception:

(Fiji Is Just) ImageJ 2.0.0-rc-69/1.52i; Java 1.8.0_66 [64-bit]; Windows 10
10.0; 320MB of 12087MB (2%)
 
java.lang.NullPointerException
        at ij.ImageStack.addSlice(ImageStack.java:105)
        at Kalman_Filter.Kalmanizer(Kalman_Filter.java:218)
        at Kalman_Filter.run(Kalman_Filter.java:89)
        at ij.IJ.runUserPlugIn(IJ.java:228)
        at ij.IJ.runPlugIn(IJ.java:192)
        at ij.Executer.runCommand(Executer.java:137)
        at ij.Executer.run(Executer.java:66)
        at java.lang.Thread.run(Thread.java:745)

Without producing any results.

Could I be doing something wrong?

Thanks in advance,

Jelle



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