Hi Sagar,
> 1. If I have a double color image (red and green), it seems that
> invariably,
> when I open the LIF set in IMAGEJ, it reverses the colors. Is there a way
> to
> fix it? It is a bit confusing at times.
>
Are you using the Bio-Formats plugin to open LIF files? If so, this problem
has been fixed in the latest trunk build of Bio-Formats (available at
http://loci.wisc.edu/ome/formats-download.html).
Regards,
-Melissa
On Fri, Aug 22, 2008 at 5:56 PM, Sagar Joshi <
[hidden email]> wrote:
> hi List -
>
> I have 2 questions -
>
> 1. If I have a double color image (red and green), it seems that
> invariably,
> when I open the LIF set in IMAGEJ, it reverses the colors. Is there a way
> to
> fix it? It is a bit confusing at times.
>
> 2. When I have 2 TIFS (generated from confocal) with two different
> objectives (and different zoom), is there a simple and accurate way to
> match
> these two images at the same zoom? for e.g. if i want to compare 2
> different
> images taken at different zooms/objectives for visually comparing their
> properties such as area
>
> Thanks.
>
> Sagar
>
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