Light-emitting Diode (LED) versus incandescent light

[Gilbert Bigras-2]

Hi all,
Does anyone know if LED source of light is a concern for microscope image acquisition?
Some new microscopes are now delivered with LED.
Spectrums of both sources of light are quite different.
Comments?

Thanks
 Gilbert Bigras, M.D., Ph.D., FRCPC (Path)

Medical Lead Edmonton Zone Immunohistochemistry Laboratory
Associate Clinical Professor
 Department of Laboratory Medicine & Pathology
 University of Alberta, Canada

Cross Cancer Institute
11560 University Avenue Edmonton, Alberta Canada T6G 1Z2
 
 Phone: [phone] Fax: [phone]

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[Chen Fengnong]

LED is a  good selection as it has lots of advantages, such as wide spectral, long use time and easy to integrate different shape.


    As to microscope deviec, I think the key is lens, maybe you should choose special lens to avoid week light transmission.


-------------------------------------------------------------------------------------------------------------
 陈丰农，博士，杭州电子科技大学   生命信息与仪器工程学院10教420
 杭州市下沙高教园区2号大街1号，310018
 
 
 Fengnong  Chen, Ph.D., Assistant Professor
 College  of Life Information Science & Instrument Engineering，Hangzhou  Dianzi University
 No.1,2nd  Street,Xiasha Higher Education Zone, Hangzhou 310018,  China
 Tel:[phone]
 Mobile:[phone]


 
 E-mail:  [hidden email]
 URL:http://lisie.hdu.edu.cn/‍

------------------ 原始邮件 ------------------
发件人: "Gilbert Bigras";<[hidden email]>;
发送时间: 2014年11月13日(星期四) 凌晨5:34
收件人: "IMAGEJ"<[hidden email]>;

主题: Light-emitting Diode (LED) versus incandescent light



Hi all,
Does anyone know if LED source of light is a concern for microscope image acquisition?
Some new microscopes are now delivered with LED.
Spectrums of both sources of light are quite different.
Comments?

Thanks
 Gilbert Bigras, M.D., Ph.D., FRCPC (Path)

Medical Lead Edmonton Zone Immunohistochemistry Laboratory
Associate Clinical Professor
 Department of Laboratory Medicine & Pathology
 University of Alberta, Canada

Cross Cancer Institute
11560 University Avenue Edmonton, Alberta Canada T6G 1Z2
 
 Phone: [phone] Fax: [phone]

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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[Gabriel Landini]

On Wednesday 12 Nov 2014 21:34:28 you wrote:
> Does anyone know if LED source of light is a concern for microscope image
> acquisition? Some new microscopes are now delivered with LED.
> Spectrums of both sources of light are quite different.
> Comments?

Hi Gilbert,
Have you seen this?

http://www.microscopy-uk.org.uk/mag/artjan14/dw-lightcompare.html


Cheers

Gabriel

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[Berg, R. Howard]

We have an LED for brightfield imaging on our dissecting scope and it has the advantage over traditional bulbs for maintaining stable spectra over different intensities.  The previous halogen color shifted depending on intensity.


R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility
Danforth Plant Science Center
975 N. Warson Rd.
St. Louis, MO 63132

ph 314-587-1261    fx 314-587-1361    
[hidden email]    www.danforthcenter.org









On Nov 13, 2014, at 12:06 AM, 浪舟 <[hidden email]>
 wrote:

> LED is a  good selection as it has lots of advantages, such as wide spectral, long use time and easy to integrate different shape.
>
>
>    As to microscope deviec, I think the key is lens, maybe you should choose special lens to avoid week light transmission.
>
>
> -------------------------------------------------------------------------------------------------------------
> 陈丰农，博士，杭州电子科技大学   生命信息与仪器工程学院10教420
> 杭州市下沙高教园区2号大街1号，310018
>
>
> Fengnong  Chen, Ph.D., Assistant Professor
> College  of Life Information Science & Instrument Engineering，Hangzhou  Dianzi University
> No.1,2nd  Street,Xiasha Higher Education Zone, Hangzhou 310018,  China
> Tel:[phone]
> Mobile:[phone]
>
>
>
> E-mail:  [hidden email]
> URL:http://lisie.hdu.edu.cn/‍
>
> ------------------ 原始邮件 ------------------
> 发件人: "Gilbert Bigras";<[hidden email]>;
> 发送时间: 2014年11月13日(星期四) 凌晨5:34
> 收件人: "IMAGEJ"<[hidden email]>;
>
> 主题: Light-emitting Diode (LED) versus incandescent light
>
>
>
> Hi all,
> Does anyone know if LED source of light is a concern for microscope image acquisition?
> Some new microscopes are now delivered with LED.
> Spectrums of both sources of light are quite different.
> Comments?
>
> Thanks
> Gilbert Bigras, M.D., Ph.D., FRCPC (Path)
>
> Medical Lead Edmonton Zone Immunohistochemistry Laboratory
> Associate Clinical Professor
> Department of Laboratory Medicine & Pathology
> University of Alberta, Canada
>
> Cross Cancer Institute
> 11560 University Avenue Edmonton, Alberta Canada T6G 1Z2
>
> Phone: [phone] Fax: [phone]
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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