Manual montage of images

[Guillermo Palchik]

Hi there,

Is there a way to manually perform a montage of images (10-bit)?
I found a command to create montages from a stack of images, but it does not work for us since it aligns images in a grid with equal overlaps. The images need to be arranged manually since our scope does not have a motorized stage, and we had to acquire the images by manually moving the stage, and therefore the x-y overlap of images differs somewhat.
I found a way to do this in Photoshop CS4 (the "collage" command), but the images must be 8-bit, and we need them to be 10 bit.
I know that this is an ImageJ forum, but since we have access to ImageJ, Photoshop CS4, and Metamorph, solutions using any of these programs are welcomed!

Thanks!
Gil

[Artsi]

>
> Hello,
>
The best thing is to download "fiji" a version of imagej with some
intresting stuff, which has two good plugins: "stich directory" which is a
life saver and it works automaticly and great even for hand moved platform,
or "mozaicJ" if you insist to do it by hand.
http://fiji.sc/wiki/index.php/Fiji
Godd luck
Hanna

>
>
> --
>
*Curiosity Killed The Cat Satisfaction Brought It Back*

[Joel Sheffield]

There is a free "demo" program called "autostitch:,
http://www.cs.bath.ac.uk/brown/autostitch/autostitch.html that may do what
you want.  I know that it is limited to jpeg images, but have no
information about bit depth requirements.  It certainly works with color
images.

Joel


On Sun, Apr 15, 2012 at 8:02 AM, Guillermo Palchik <[hidden email]>wrote:

> Hi there,
>
> Is there a way to manually perform a montage of images (10-bit)?
> I found a command to create montages from a stack of images, but it does
> not work for us since it aligns images in a grid with equal overlaps. The
> images need to be arranged manually since our scope does not have a
> motorized stage, and we had to acquire the images by manually moving the
> stage, and therefore the x-y overlap of images differs somewhat.
> I found a way to do this in Photoshop CS4 (the "collage" command), but the
> images must be 8-bit, and we need them to be 10 bit.
> I know that this is an ImageJ forum, but since we have access to ImageJ,
> Photoshop CS4, and Metamorph, solutions using any of these programs are
> welcomed!
>
> Thanks!
> Gil
>

--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs

[Pavel Tomancak]

Try stitching in Fiji

http://fiji.sc/wiki/index.php/Image_Stitching

PAvel

On Apr 15, 2012, at 2:02 PM, Guillermo Palchik wrote:

> Hi there,
>
> Is there a way to manually perform a montage of images (10-bit)?
> I found a command to create montages from a stack of images, but it  
> does not work for us since it aligns images in a grid with equal  
> overlaps. The images need to be arranged manually since our scope  
> does not have a motorized stage, and we had to acquire the images by  
> manually moving the stage, and therefore the x-y overlap of images  
> differs somewhat.
> I found a way to do this in Photoshop CS4 (the "collage" command),  
> but the images must be 8-bit, and we need them to be 10 bit.
> I know that this is an ImageJ forum, but since we have access to  
> ImageJ, Photoshop CS4, and Metamorph, solutions using any of these  
> programs are welcomed!
>
> Thanks!
> Gil

[Albert Cardona-2]

On Apr 15, 2012, at 8:02 AM, Guillermo Palchik <[hidden email]> wrote:

> Hi there,
>
> Is there a way to manually perform a montage of images (10-bit)?
> I found a command to create montages from a stack of images, but it does not work for us since it aligns images in a grid with equal overlaps. The images need to be arranged manually since our scope does not have a motorized stage, and we had to acquire the images by manually moving the stage, and therefore the x-y overlap of images differs somewhat.
> I found a way to do this in Photoshop CS4 (the "collage" command), but the images must be 8-bit, and we need them to be 10 bit.
> I know that this is an ImageJ forum, but since we have access to ImageJ, Photoshop CS4, and Metamorph, solutions using any of these programs are welcomed!


If you need to rotate, scale or affine transform the images, try TrakEM2:

http://fiji.sc/TrakEM2

>

Albert

[Guillermo Palchik]

Thanks all for all the suggestions!
Hanna, MosaicJ worked like a charm!!!! Thanks!
Gil

On Apr 15, 2012, at 8:24 AM, Hanna Artsi wrote:

>>
>> Hello,
>>
> The best thing is to download "fiji" a version of imagej with some
> intresting stuff, which has two good plugins: "stich directory" which is a
> life saver and it works automaticly and great even for hand moved platform,
> or "mozaicJ" if you insist to do it by hand.
> http://fiji.sc/wiki/index.php/Fiji
> Godd luck
> Hanna
>
>>
>>
>> --
>>
> *Curiosity Killed The Cat Satisfaction Brought It Back*

--
Guillermo Palchik
Ph.D. Candidate - Interdisciplinary Program in Neuroscience
Georgetown University Medical Center
Research Building Room W 217
3970 Reservoir Rd. NW, Washington, DC 20007
Lab: 202-687-7825