Measuring area without intensity

[Roseann]

I have what I think is a simple question.  I have a green image and would like to measure the area of the image only (not the intensity).  Is there a way of doing this without manually circling each image?

I'd very much appreciate everyone's help.

[dscho]

Dear SUBSCRIBE IMAGEJ Roseann,

On Mon, 10 Jan 2011, SUBSCRIBE IMAGEJ Roseann wrote:

> I have what I think is a simple question.

It is good that you think this is a simple question, otherwise how would
people on this list be able to answer it?

> I have a green image and would like to measure the area of the image
> only (not the intensity).  Is there a way of doing this without manually
> circling each image?

Do you mean the whole image? I.e. the rectangle that is the bounding box?
Then the text on top of the image in the image window should provide the
information (iff the image is calibrated).

> I'd very much appreciate everyone's help.

Including... really everyone? :-)

Ciao,
Johannes

[Jacqueline Ross]

Dear Roseann,

Further to Johannes' reply, if you just want to measure the "green" area
in the image, then you just need to define that area by thresholding
(Image - Adjust threshold) or by making the image binary (Process - Make
Binary) and then select AREA as one of the parameters you want to
measure (Analyze - Set Measurements). If you are using a manual
threshold, then select "Limit to Threshold", before measuring.

You should calibrate the image first so that your results are in the
correct units, e.g. microns squared.

It's easier if you can work in greyscale, e.g. make the image 8bit first
but otherwise you can use the built-in colour thresholding if necessary.

Kind regards,

Jacqui

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
SUBSCRIBE IMAGEJ Roseann
Sent: Tuesday, 11 January 2011 1:18 p.m.
To: [hidden email]
Subject: Measuring area without intensity

I have what I think is a simple question.  I have a green image and
would like to measure the area of the image only (not the intensity).
Is there a way of doing this without manually circling each image?

I'd very much appreciate everyone's help.