Measuring individual pixel intensities inside ROIs

[Shiva A. Portonovo]

Hello,

I am interested in selecting several ROIs in an image, and then acquiring
intensity data for pixels inside of the ROIs, instead of just the average,
min, or max value.  I know that saving the file as a text image returns the
grey scale data, but I have no way of identifying the ROIs.  Does anyone
know of a method for doing this?

Incidentally, I'm interested in doing this for stacks, so it would be really
great if different pixel value intensities inside of an ROI could be
recorded for individual slices in a stack as well.

Thank you very much for any assistance,
Shiva

[audrey karperien]

Check out the code for FracLac; it does what you describe.
 

"Shiva A. Portonovo" <[hidden email]> wrote:
  Hello,

I am interested in selecting several ROIs in an image, and then acquiring
intensity data for pixels inside of the ROIs, instead of just the average,
min, or max value. I know that saving the file as a text image returns the
grey scale data, but I have no way of identifying the ROIs. Does anyone
know of a method for doing this?

Incidentally, I'm interested in doing this for stacks, so it would be really
great if different pixel value intensities inside of an ROI could be
recorded for individual slices in a stack as well.

Thank you very much for any assistance,
Shiva