Noname,
this is a cross-post from the ImageJ-Forum.
<
http://forum.imagej.net/t/modified-ratio-calculation-of-two-rois-within-one-image/513/2>
BTW, what "BioVoxxel" has suggested there is not what you have asked for.
Best
Herbie
:::::::::::::::::::::::::::::::::::::::
Am 14.12.15 um 14:24 schrieb lgentemann:
> Dear list,
>
> I am looking for a way to calculate the intensity ratio out of two ROIs
> within one image (see exemplary image below). As my goal will be to
> calculate the polarization anisotropy out of those two ROIs, I moreover
> don't want to calculate only a usual ratio like r=I1/I2 but rather a
> modified ratio like r=(I1-I2)/(I1+2*I2) with I1 and I2 being the pixel
> intensities of both ROIs. As I wish to do this while live recording using
> MicroManager, I want to avoid to just duplicate both ROIs as single images
> and doing the calculation afterwards.
> So far, I've tried using the Ratio Plus plugin but this seems to not be able
> to calculate a ratio out of two ROIs instead of out of two images. Also, I
> couldn't find a possibilty to modify the ratio-equation the way I wish to.
> I hope any of you might have some helpful tips on this issue.
> Thanks!
>
> Signal_-_Image.jpg
> <
http://imagej.1557.x6.nabble.com/file/n5015235/Signal_-_Image.jpg>
>
>
>
> --
> View this message in context:
http://imagej.1557.x6.nabble.com/Modified-ratio-calculation-of-two-ROIs-within-one-image-tp5015235.html> Sent from the ImageJ mailing list archive at Nabble.com.
>
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