Muscle

[Petkov, Daniel]

Hello,

We have small project for which macrophages in skeletal muscle have to be enumerated. Target cells are stained with surface markers with green or red color and muscle sections with DAPI. The muscle has high autofluorescence and targeted cells have elongated shape.

Can ImageJ be used to enumerate these cells in muscle?

Thank you for the help,
Daniel

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[Jacqueline Ross]

Hi Daniel,

I'm sure someone will be able to help but we need to see an image! Could you please post one somewhere?

Cheers,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Petkov, Daniel
Sent: Saturday, 5 September 2015 2:58 a.m.
To: [hidden email]
Subject: Muscle

Hello,

We have small project for which macrophages in skeletal muscle have to be enumerated. Target cells are stained with surface markers with green or red color and muscle sections with DAPI. The muscle has high autofluorescence and targeted cells have elongated shape.

Can ImageJ be used to enumerate these cells in muscle?

Thank you for the help,
Daniel

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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ImageJ mailing list: http://imagej.nih.gov/ij/list.html